Procedures are described for the purification of calf thymus nuclei using mild hypotonit shock to break intact cells, and layering techniques to remove cytoplasmic debris. Ficolc (a high polymer of sucrose) was dissolved in isotonic sucrose to give dense solutions suitable for gradient centrifugation. The method yields nuclei which can incorporate amino acids in vitro. Thymus nuclei isolated un...

Abstract—Developing our knowledge of when pineapple roots grow can lead to improved water, fertilizer applications, and more precise culture management. This paper presents current understanding of morphological traits in pineapple roots, highlighting studies using incubation periods and various solid MS media treated with different sucrose concentrations and pH, which directly assess in vitro ...

Sucrose transporters (SUTs) are known to play critical roles in the uptake of sucrose from the apoplast in various steps of sugar translocation. Because developing pollen is symplastically isolated from anther tissues, it is hypothesized that SUTs are active in the uptake of apoplastic sucrose into pollen. To investigate this possibility, a comprehensive expression analysis was performed for me...

Tonoplast vesicles isolated from stalk parenchyma tissue of sugarcane plants transport sucrose via a uridine diphosphate glucose (UDPGlc)-dependent group translocator. No sucrose transport via an ATP-dependent system could be detected. The products of UDPGlc uptake in the vesicles were sucrose and sucrose phosphate which, upon hydrolysis with alkaline phosphatase and invertase, showed that both...

Bestatin, a dipeptide analog, is a potent aminopeptidase inhibitor of bacterial origin. We have previously shown that bestatin inhibits cytosolic exopeptidases in mammalian cells, and results in the accumulation of di- and tripeptide intermediates in cellular protein degradation. Our primary interest is the uptake of bestatin in liver and muscle, 10 min after its intravenous injection into mice...

ABSTRACT- Chrysanthemum (Dendranthema grandiflorum) is one of the most important ornamental plants used as cut flower, flowering pot plant and groundcover worldwide. In this research, the effects of preservative solutions containing thymol (75 and 125 mg -L), menthol (75 and 125 mg L-1) and eugenol (75 and 125 mg L-1) on the quality and vase-life of chrysanthemum cut flowers at 3±1ºC and 75-80%...

In micropropagation of lily, preferably bulblets should be produced: Because bulblets are compact and robust, they are much easier to handle and to plant in soil than shoots. In this review, the various factors that determine bulblet growth in vitro are discussed. Gibberellins, jasmonates (JA) and abscisic acid (ABA) are the major identified plant growth regulators (PGRs) for storage organ form...

The conjugative plasmid pUR400 determines tetracycline resistance and enables cells of Escherichia coli K-12 to utilize sucrose as the sole carbon source. Three types of mutants affecting sucrose metabolism were derived from pUR400. One type lacked a specific transport system (srcA); another lacked sucrose-6-phosphate hydrolase (scrB); and the third, a regulatory mutant, expressed both of these...