We describe a technique that uses biocompatible chemical agents to enhance both the sensitivity and the resolution of in vivo imaging with low-level light-emitting probes. We demonstrate experimentally, with chemiluminescence (CL) imaging in vitro as an example, that the detected intensity of CL from treated 3-mm-thick skin tissue is approximately fivefold stronger than that from untreated skin...

We show that phase-conjugation by three-wave mixing allows turbidity suppression through biological tissues with thicknesses up to 5 mm, at a near-infrared wavelength included in the therapeutic window. Because of the ultrafast character of the imaging process, a motion of the tissue, which mimics in vivo imaging, can be exploited to significantly improve the signal-to-noise ratio and the resol...

To gain insight into the dendritic structural changes that occur within minutes of ischemia, as well as circuit-level plasticity that occurs over days to weeks, it is necessary to devise stroke models for anesthetized transgenic mice that are compatible with in vivo imaging methods. In particular, we are interested in examining events during the first few minutes of ischemia that are associated...

Two mitochondria-targeted real-time probes were presented, which could selectively respond to hypochlorite over other ROS. Meanwhile, the "off-on" probes could be successfully applied in the in vivo imaging of hypochlorite in living mice.

Among the various anions, only cyanide induced the revival of fluoresecence of -Cu(2+) resulting in "Off-On" type sensing of cyanide, which can be monitored at pH 7.4 in 100% aqueous system, and has been applied to a microfluidic platform, in which fluorescent sensor -Cu(2+) displayed green fluorescence upon the addition of cyanide, the in vivo imaging of cyanide using Caenorhabditis elegans.

Herein, we describe a fast and robust method for achieving (68)Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels-Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.

Forages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using vitro vivo methods this study investigated effect pre-treating Smutsfinger hay for 24 hours a mixture enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% 100% xylanase c...

Two experiments were conducted to determine the effects of enzyme complex on in vitro dry matter (DM) digestibility for feed ingredients. The objective of experiment 1 was to screen feed ingredients that can be effective substrates for an enzyme complex, mainly consisted of β-pentosanase, β-glucanase and α-amylase, using in vitro digestibility methods. In experiment 1, the test ingredients were...

In this study the values of true digestibility of DM, OM, CP, NDF and starch determined either by the nylon capsule method or conventional in vivo method were compared. Four intact crossbred dairy cows (mean milk yield 21.9 kg/d) were used in two experimental periods. TMR consisted of maize silage, lucerne hay and concentrate. Nylon capsules (external diameter 10 mm) were made of nylon cloth (p...