The incidence of Aspergillus fumigatus infections has become more frequent as a consequence of widespread immunosuppression. At present, the number of available antifungal agents in the clinic is limited, and most of them, such as itraconazole (ICZ), are toxic and show resistance. Berberine (BER) is a plant alkaloid used in the clinic mainly for alimentary infections. We have used BER and ICZ t...

The Mre11/Rad50/Xrs2 (MRX) complex in Saccharomyces cerevisiae has well-characterized functions in DNA double-strand break processing, checkpoint activation, telomere length maintenance and meiosis. In this study, we demonstrate an involvement of the complex in the base excision repair (BER) pathway. We studied the repair of methyl-methanesulfonate-induced heat-labile sites in chromosomal DNA i...

During mammalian base excision repair (BER) of lesion-containing DNA, it is proposed that toxic strand-break intermediates generated throughout the pathway are sequestered and passed from one step to the next until repair is complete. This stepwise process is termed substrate channeling. A working model evaluated here is that a complex of BER factors may facilitate the BER process. FLAG-tagged ...

Blossom-end rot (BER) is a physiological disorder related to calcium (Ca) deficiency in tomato fruits. However, adequate Ca supply not sufficient inhibit the BER incidence. The Ni-Ca interact...

The flaw in 5G orthogonal frequency division multiplexing (OFDM) becomes apparent high-speed situations. Because the doppler effect causes shifts, orthogonality of OFDM subcarriers is broken, lowering both their bit error rate (BER) and throughput output. As part this research, we use a novel design that combines massive multiple input output (MIMO) weighted overlap add (WOLA) to improve perfor...

To elucidate the nature of the cross-talk between the p53 protein and the DNA repair machinery, we have investigated the relationship between the two throughout the cell cycle. Base excision repair (BER) was analyzed in cell cycle phase-enriched populations of lymphoid cells expressing wild-type p53. Our study yielded the following novel findings: (a) BER exhibited two distinct peaks of activit...

Base excision repair (BER) is one of the cellular defense mechanisms repairing damage to nucleoside 5'-monophosphate residues in genomic DNA. This repair pathway is initiated by spontaneous or enzymatic N-glycosidic bond cleavage creating an abasic or apurinic-apyrimidinic (AP) site in double-stranded DNA. Class II AP endonuclease, deoxyribonucleotide phosphate (dRP) lyase, DNA synthesis, and D...

ÐMetal matrix composites (MMCs) can be synthesized by aligning metal coated ceramic ®bers in a shaped container and applying pressure at elevated temperatures. The high stresses created at the contacts between neighboring ®bers cause inelastic matrix ̄ow that ®lls inter®ber voids, reduces inter®ber separations and results in composite densi®cation. The rate of densi®cation depends on the contac...

The base excision DNA repair (BER) pathway known to occur in Caenorhabditis elegans has not been well characterized. Even less is known about the DNA polymerase (pol) requirement for the gap-filling step during BER. We now report on characterization of in vitro uracil-DNA initiated BER in C. elegans. The results revealed single-nucleotide (SN) gap-filling DNA polymerase activity and complete BE...