An attempt was made to standardize an in-house immuno-enzyme assay for measuring antibodies of infectious bursal disease virus (IBDV) in chicken. The test was performed after coating plates with ELISA antigens prepared by two methods. Antigen “A” was prepared from infectious bursal disease virus infected chicken embryo fibroblasts culture by concentration with dialysis against PEG-6000 while an...

Pemphigus (from the Greek pemphix, meaning bubble or blister) is a blistering disease involving the skin and mucous membrane. The disease is characterized histologically by acantholysis which means loss of adhesion between keratinocytes, and immuno-pathologically by the presence of immunoglobulin directed towards the cell surface of kerat inocytes . 1 The landmark ar t ic le in the understandin...

An indirect format enzyme-linked immuno-sorbent assay (ELISA) on graphite rods ( 0.8 mm x 20 mm) and, for comparison, on microtiter plates has been developed against terbuthylazine. For this purpose, a series of 2-aminoalkyl-4-chloro-6-terbuthyl-s-triazine-2,6-diamine ELISA haptens with alkyl spacer lengths of 2, 4, 6, and 8 CH2 groups has been synthesized. The graphite rods or the microtiter p...

protein quantitation that obtains precise and sensitive measurements of specific target analytes in complex biological samples. Prior to performing MRM, many peptide analytes require an enrichment step to achieve sufficient sensitivity in the assay. Coupling a peptide immunoaffinity enrichment step to MRM (i.e. an immuno-MRM assay) is effective for most analytes and provides high sensitivity an...

The virus causing hydropericardium syndrome was isolated in chicken embryo liver (CEL) cell culture from livers obtained from naturally infected broilers. The cytopathic effects characterized by rounding and degeneration of cells were visible 36 hr post infection in first passage. At 4th passage level, the infectivity titre was 5.24 log10 TCID50/ml. In May-Grunwald and Giemsa stained cells, bas...

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains secrete toxins that are major virulence factors and diagnostic targets, but some STEC strains secrete Stx in amounts that cannot be detected using conventional cell cytotoxicity or immunological assays. Therefore, there is an urgent need for more-sensitive Stx detection methods. We describe the development of an assay that can detect l...