A sensitive, specific, and simple method for determining serum or urine arylesterase (EC 3.1.1.2) is described. The enzyme acts on phenyl acetate to release phenol, which produces a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide. Arylesterase, a thiol enzyme, is reactivated by 2-mercaptoethanol and by cysteine, but not by reduced glutathione. Calcium is indispensable to...

was suggested by Rotter (1937). He observed that when 0.01 c.cm. of a N/400 solution of the dye 2 : 6-dichlorophenol indophenol was injected intradermally, the colour of the dye disappeared gradually. When methyl blue was similarly injected, its colour remained unchanged, which showed that the decolorization of dichlorophenol indophenol was not due to resorption but to reduction. Rotter (1938) ...

Although there is a considerable amount of information on the effect of light intensity on O2 evolution and, to a lesser extent, COz assimilation in intact plants (l), there are few reports on the effect of light intensity on the photosynthetic processes known to occur in the isolated chloroplast and chloroplast fragments. Isolated chloroplasts have the ability to assimilate CO2 (‘2, 3), and ch...

cyanobacteria were collected and identified in five paddy fields with different soil textures of golestan province in iran. it was determined that nostoc ellipsosporum and nostoc muscorum were dominant in all sites among 20 species. to assess the ability of atmospheric nitrogen fixation by the species, their pure cultures were provided by growing on bg-11 solid culture medium. subsequently, eac...

During growth, Acetabularia mediterranea requires the action of blue light to maintain high rates of photosynthesis. In the present study, blue light-dependent alterations of the photosynthetic apparatus, which can be detected by analysis of light-saturation curves and by measurements of partial reactions of the photosynthetic electron transport chain, are described. Light-saturation curves of ...

A bstract. Four mutant strains of Euglena gracilis have been isolated after treatment of wild type cells with ultraviolet light or the chemtical mutagen nitrosoguanidine. None of the mutants is capable of autotrophic growth or photosynthetic carbon dioxide fixation. The mutant strains contain normal amounts of the enzymes of the reductive pentose phosphate cycle and are qualitatively similar to...

13 independent mouse-human somatic cell hybrid clones derived from beta-propiolactone-inactivated Sendai stimulated cell fusion of human cells with mouse cells were tested for their sensitivities to human and mouse interferon. All of them were protected by mouse interferon and only six of the clones were protected by both human and mouse interferon. Only the six that were protected by human int...