A series of substituted dihydro-1, 2-oxazole benzopyran-2-one (SR1-SR6) were synthesized through the intermediate chalcones and characterized using spectral analysis. Compounds docked with receptor DNA Gyrase B (PDB code: 5L3J) to know its interaction binding energy; ranges -3.38 -2.15 kcal/mol. Further these compounds tested for antibacterial activity tube dilution method MIC values observed; ...