SRC kinases and PKR are intracellular protein kinases, which play key roles in intracellular viral replication. In this research, the effect of SRC kinase inhibition and PKR activation and inhibition on replication of coxsakievirus (CVB3), an entrovirus of the family picornaviridae – causative agents of fatal myocarditis, was studied. Vero and Hela cells were cultured and infected with CVB3 in ...

Introduction: we have recently reported the presence of two potassium currents with 598 and 368 pS conductance in the rough endoplasmic reticulum (RER) membrane. The 598 pS channel was voltage dependent and ATP sensitive. However, the 368 pS channel was rarely observed and its identity remained obscure. Since cationic channels in intracellular organelles such as mitochondria and RER play imp...

In this study, the biosynthesis of gold nanoparticles by Penicillium chrysogenum, isolated from Ahar copper mine, was investigated. The gold nanoparticles were synthesized by reducing the aqueous gold ions using the culture supernatant of the filamentous fungi. The UV–vis spectrum displayed a characteristic peak at 532 nm that is very specific for gold nanoparticles. The XRD spectrum confirmed ...

It is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate M. aviumand M. intracellulare from one another and from M. scrofulaceum strains. The purpose of this study was to evaluate the extent to which 16S rRNA sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

Background: Endometriosis is a complex disease that profoundly affects the quality of life in many women. This disease affects roughly one in ten women of reproductive age. Endometriosis induces a variable amount of inflammatory reaction in pelvic environment. An active immune system needs to recognize these inflammatory agents. Rapid innate immune system defenses against infections involve the...

Intracellular pathogens reside in specialised compartments within the host cells restricting access of antibiotics. Insufficient intracellular delivery antibiotics along with several other resistance mechanisms weaken efficacy current therapies. An alternative to antibiotic therapy could be bacteriophage (phage) therapy. Although phage has been practice for a century against various bacterial i...