نتایج جستجو برای: inverse pcr

تعداد نتایج: 264411  

Journal: :Annals of medical research 2022

ABSTRACT Aim: Corona virus disease 2019 (COVID-19) mainly affects the lungs. However, other organs are affected as well. This article aimed to evaluate effects of coronavirus infection on liver density in addition laboratory findings. Methods: Biochemical parameters and non-contrast tomography images patients who presented pandemic clinic were studied retrospectively. Densities right left lobes...

روزافزای, نرگس, زینلی, سیروس, کریمی پور, مرتضی, کوکبی, لیلا,

  زمینه و هدف : هموفیلی A یک اختلال انعقادی وابسته به جنس مغلوب است که در اثر وقوع ناهنجاری های گوناگون در ژن فاکتور هشت انعقادی حادث می گردد . وارونگی اینترون 22 در50-45% ازموارد مسبب نوع شدید بیماری است. علاوه بر این وارونگی اینترون 1 نیز در بیش از 5% موارد مسئول ایجاد هموفیلی A شدید محسوب می گردد. هدف از این مطالعه ارزیابی دقیق وارونگی اینترون1 ژن فاکتور هشت انعقادی با استفاده از روش IS-PCR(...

Journal: :Robotics and Autonomous Systems 2009
Stefan Staicu

Recursive modelling for the kinematics and dynamics of the known 3-PRR planar parallel robot is established in this paper. Three identical planar legs connecting to the moving platform are located in a vertical plane. Knowing themotion of the platform,we develop first the inverse kinematics and determine the positions, velocities and accelerations of the robot. Further, the principle of virtual...

2015
Yousry Hawash M. M. Ghonaim Ayman S. Al-Hazmi

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it should be properly monitored by an inhibition control. In this study, a cloning vector harboring a modified target DNA sequence (≈375 bp) was constructed to be used as a competitive internal amplifica...

Journal: :Journal of applied physiology 2013
Bernard Korzeniewski Jerzy A Zoladz

The computer model of the bioenergetic system in skeletal muscle, developed previously, was used to study the effect of the characteristic decay time of the parallel activation of oxidative phosphorylation [τ(OFF)] during muscle recovery on the muscle oxygen consumption rate (Vo2) and phosphocreatine (PCr) work-to-rest transition (off)-kinetics and on the relationship between the Vo2 and PCr re...

Journal: :FEMS yeast research 2014
Lucia Oro Severino Zara Francesca Fancellu Ilaria Mannazzu Marilena Budroni Maurizio Ciani Francesca Comitini

Tetrapisispora phaffii produces a killer toxin known as Kpkt that has extensive anti-Hanseniaspora/Kloeckera activity under winemaking conditions. Kpkt has a β-glucanase activity and induces ultrastructural modifications in the cell wall of sensitive strains, with a higher specific cytocidal activity and a selective action towards target yeast cells. In this study, a two-step PCR-based approach...

Journal: :Bioscience, biotechnology, and biochemistry 2006
Yuji Tasaki Hiromichi Yoshikawa Hiroto Tamura

Octylphenol polyethoxylate (OPEO(n)) biodegradation by Pseudomonas putida S-5 under aerobic conditions is initiated by the oxidation of its terminal alcohol group by alcohol dehydrogenase. A DNA fragment, containing an alcohol dehydrogenase gene (adh1), was isolated using a combination of degenerate PCR and inverse PCR. The predicted translation product of adh1 showed significant sequence simil...

Journal: :BioTechniques 2000
J Phan K Reue M Péterfy

Microsatellites (MS) are excellent genetic markers because of their prevalence in eukaryotic genomes, ease of typing by PCR and high degree of polymorphism. MS-based genetic maps of the human and mouse genomes have been constructed with average intermarker interval sizes of 1.6 cM and 0.42 cM, respectively (1,6). While these maps are essential resources for gene mapping, their resolution may no...

2016
Sally Partridge Béatrice Berçot Érica L. Fonseca Ana Carolina Paulo Vicente

The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF), a short 5’ untranslated region (UTR) and a 3’ recombination site ( ). Fused gene cassettes are generated attC by partial or total loss of the from the first cassette in an array, creating, in attC some cases, a fusion with the ORF from the next cassette. These structures are r...

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