Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at roo...

This two-site IRMA includes specific monoclonal antibodies for measuring skeletal alkaline phosphatase (B-ALP) in human serum. Assay calibration is based on mass units (micrograms per liter) and was established with purified B-ALP from a human osteosarcoma cell line, SAOS-2. Precision studies demonstrated intra- and interassay CVs of 3-5% and 5-7%, respectively. Relative reactivity studies show...

The objective of this study is to understand how Cuba responds extreme weather events, which can help identify and disseminate good public health practice. design an observational using routinely collected mortality data. National daily counts after severe hurricanes arrived on the landmass since 1990 were compared with baseline values. Incidence rate ratios during hurricane for four weeks afte...

Eleven commercial kits for serum ferritin have been compared. Seven kits used RIA and four IRMA methodology. Seven kits used human liver ferritin, two human spleen ferritin and two undefined human ferritin as standard material. Incubation times varied between 30 minutes and 36 hours. Only four kits gave normal ranges determined with the kit in question. All values were compared with the kit in ...

A sensitive immunoradiometric assay (IRMA) for human growth hormone (GH) using monoclonal antibodies was evaluated for clinical application. The detection limit of GH in serum was 0.05 ng/ml using 0.1 ml of serum. The specificity and assay precision were satisfactory. GH levels in serum determined by the IRMA were well correlated to those determined by the sensitive enzyme immunoassay or the co...

Because of the lack of mammography databases with a large amount of codified images and identified characteristics like pathology, type of breast tissue, and abnormality, there is a problem for the development of robust systems for computer-aided diagnosis. Integrated to the Image Retrieval in Medical Applications (IRMA) project, we present an available mammography database developed from the u...

Content-based medical image retrieval can support diagnostic decisions by clinical experts. Examining similar images may provide clues to the expert to remove uncertainties in his/her final diagnosis. Beyond conventional feature descriptors, binary features in different ways have been recently proposed to encode the image content. A recent proposal is “Radon barcodes” that employ binarized Rado...

AIMS To study the effect of in-utero alcohol exposure on the insulin-like growth factor axis (IGF) and leptin during infancy and childhood, considering that exposed children may exhibit pre- and postnatal growth retardation. METHODS We prospectively identified heavily drinking pregnant women who consumed on average 4 or more drinks of ethanol per day (≥ 48 g/day) and assessed growth in 69 of ...