An isocratic sensitive and precise reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination and quantification of hydrocortisone in controlled-release and conventional (tablets and injections) pharmaceutical preparations. Chromatographic separation was achieved on an ODS (C18), 5 μm, 4.6 × 150 mm, with an isocratic elution using a f...

The aim of pharmaceutical analysis is to obtain the necessary qualitative and quantitative information about the investigated sample. HPLC is the dominant separation technique in modern pharmaceutical and biomedical analysis because it results in highly efficient separations and in most cases provides high detection sensitivity. Chromatographic optimization procedures are becoming more multidis...

ThaiHuu Nguyen, Renjun Pei, Donald W. Landry, Milan N. Stojanovic and Qiao Lin Department of Mechanical Engineering, Columbia University, New York, NY 10027; Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032 Abstract: We present a microfluidic aptameric biosensor, or aptasensor, for selective detection of clinically ...

Two reversed-phase liquid chromatographic (RP-LC) methods are described for the determination of two binary mixtures of hypoglycemic agents. In the first method, vildagliptin (VDG) was determined in the presence of 3-amino-1-adamantanol (AAD), a synthetic intermediate and impurity of VDG. In the second method, pioglitazone hydrochloride (PGZ) and metformin hydrochloride (MET) were simultaneousl...

A simple, sensitive isocratic reversed-phase HPLC method for detecting and quantifying ciprofloxacin in pharmaceutical dosage forms has been developed validated according to ICH guidelines. In the method, separation was achieved on a C18 column with mobile phase acetonitrile-water at ratio of (80:20). The pH adjusted 2.7 using 85% phosphoric acid ultraviolet detection 275.0 nm. flow rate 1.7 mL...

The objective of the current study was to develop and validate a simple, precise and accurate isocratic stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) assay method for the determination of spironolactone and furosemide in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on an SGE 150 × 4.6 mm SS Wakosil II 5C8RS 5-μm column using...

Several isocratic separations for the determination of 20 steroids (STER) in animal feeding water samples (AFWS) from drinking-trough by LC using a mobile phase ACN/H(2)O (35:65 v/v) and different RP columns (Hypersil C18, Gemini C18 (GM), Purospher Star C18, Synergi Max C12, and Synergi Fusion) and UV detection were obtained. The elution order was the same: a first group of corticoids (CC) was...

This article describes the use of a new prototype column hardware made with 1.5 mm internal diameter (i.d.) and demonstrates some benefits over 1.0 i.d. micro-bore column. The performance 2.1, columns were systematically compared. With column, loss apparent efficiency can be significantly reduced compared to in both isocratic gradient elution modes. In end, is almost comparable 2.1 from peak br...

The aims were to develop a liquid chromatographic (LC) method with ultraviolet detection (UVD) for O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) O-oximes of common aldehydes and ketones, and to define the steric limits of the synthetic reaction used to make the PFBHA O-oxime standards for gas chromatographic (GC) and LC methods. Ten new O-oximes were synthesized with the ne...

A simple, specific and sensitive high-performance liquid chromatographic (HPLC) method for determination of S (-) Amlodipine Besylate (SAB) and its related substance i.e. R (+) Amlodipine Besylate (RAB) in tablet formulation was developed and validated. The isomer separation was carried out using simple isocratic reversed-phase HPLC method using a chiral column. Isocratic elution at a flow rate...