Genetic evidence from mutant mice suggests that alpha(2)-HS glycoprotein/fetuin-A (Ahsg) is a systemic inhibitor of precipitation of basic calcium phosphate preventing unwanted calcification. Using electron microscopy and dynamic light scattering, we demonstrate that precipitation inhibition by Ahsg is caused by the transient formation of soluble, colloidal spheres, containing Ahsg, calcium, an...

background epidermal growth factor (egf) plays a fundamental role in the healing of wounds relating to skin damage, the cornea, and the gastrointestinal tract. objectives the aim of this study is the cloning, expression, and purification of recombinant human egf (rhegf), and an assessment of its activity. materials and methods in the present experimental study, a synthetic pet28a (+) -hegf cons...

Objective: The aim of this study was to obtain protein isolate from sacha inchi using alkaline pH at different pHs of precipitation with water and salt and to analyze protein isolate with electrophoresis. Methods: Sacha inchi protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using electrophoresis native-polyacrylamide gel electrophore...

Phosphoenolpyruvate carboxykinase (EC 4.1.1.32) has been isolated and purified from the cytosol fraction of rat liver to give a specific activity of 14 to 16 moles of bicarbonate tied per min per mg of protein. The enzyme is homogeneous on disc electrophoresis and Sephadex G-100 chromatography and has a molecular weight of 74,500 with an isoelectric point of 5.04. Antibodies prepared in rabbits...

An experimental procedure was developed to characterize the solubility of the soybean protein fractions close to the isoelectric point. The results show that the 7S fraction is precipitated in a much narrower range of pH values than the 11S fraction. Surprisingly, the addition of salt to the solutions leads to increased solubility of proteins, unlike the common "salting out" effect generally ex...

Transmission of specific immunoglobulins from mothers to their offspring via the egg is a common phenomenon in egg-laying vertebrates but the occurrence of this phenomenon in reptiles, especially in colubrid snakes, has not been proven until recently. Thus, the essential biochemical characteristics of antibodies deposited in eggs of Elaphe guttata (Colubridae, Serpentes) were studied after isol...

A procedure is described for the purification of cyclic adenosine 3’,5’-monophosphate receptor protein (CRP) from Escherichia coli involving chromatography on DEAE-cellulose and phosphocellulose, (NH4)&04 precipitation, and filtration on Sephadex G-100. The preparation appears to be homogeneous as determined by sedimentation equilibrium studies, isoelectric focusing, and amino-terminal amino ac...

Traditionally, human milk proteins are identified largely by reference to bovine milk. Hence, to identify the major proteins in human milk, we subjected human and bovine milk, in parallel, to high-resolution two-dimensional electrophoresis. Isoelectric precipitation at pH 4.6 was our criterion for distinguishing whey proteins from those of the casein complex. The alpha- and beta-caseins were id...

Electrophoretic patterns of mixtures of ovalbumin and yeast nucleic acid indicate that the constituents migrate independently of each other in buffer solutions of 0.1 ionic strength and at pH values somewhat higher than the isoelectric point of the protein. In the isoelectric region, however, the patterns from the two sides of the channel exhibit asymmetries that can be explained by assuming th...