Lactate dehydrogenase C, an isoenzyme composed of C polypeptide subunits and found only in mature testes and spermatozoa, differs kinetically, chemically and immunologically from the five common isoenzymes of lactate dehydrogenase, each of which is a tetramer of A and/or B subunits. In the rat lactate dehydrogenase C exists in two molecular forms, isoenzymes C4 and A1C3. In addition to these tw...

We have studied five patients who have exhibited an unusual alkaline phosphatase isoenzyme (ALP EC 3.1.3.1) migrating in an ultrafast position electrophoretically on cellulose acetate. This ALP isoenzyme has been identified in patients with benign and malignant liver diseases. In addition, a number of these patients exhibited a regular ALP liver isoenzyme and a fast (preliver) ALP isoenzyme in ...

Evidence of acute pancreatitis was sought in 35 patients with fulminant hepatic failure. Total amylase was raised in 22 patients and isoenzyme separation showed a distinct P3 isoenzyme (indicative of pancreatitis) in 14. In four patients with marked hyperamylasaemia (greater than 1000 U/l) the predominant isoenzyme was the salivary fraction. Pancreatic lipase was abnormally raised (greater than...

An electrophoretic variant of lactate dehydrogenase (LD) M(A) subunit was discovered in a female patient with chest pain. Her LD activity in serum was within the normal reference interval, and analysis of her LD isoenzyme pattern showed an abnormal migration indicating a fast-type LD-M(A) subunit variant. DNA analysis of the mutant LD-M gene detected a single base substitution, an A to G transi...

Effect of salinity on malate dehydrogenase activity was studied.Pea root tips contain 2 different malate dehydrogenases. One is located in the particulate, mitochondrial fraction, the other in the soluble, cytoplasmic fraction. Both can act when coupled with either NAD or NADP.Growing plants in Na(2)SO(4) salinated medium did not affect the pattern of the malate dehydrogenases in the root tips....

Diabetes mellitus is a group of metabolic disorders that share with symptoms hyperglycemia led to elevated free radical activity. Hyperglycemia linked higher level (ADA), which one the factors cause oxidative stress by creating reactive oxygen species (ROS), leads insulin resistance. This study aimed determine relationship between serum total adenosine deaminase(TADA) and its isoenzyme (ADA1and...