The antibacterial effect of the soybean phytoalexin glyceollin was assayed using a liquid microculture technique. Log-phase cells of Bradyrhizobium japonicum and Sinorhizobium fredii were sensitive to glyceollin. As revealed by growth rates and survival tests, these species were able to tolerate glyceollin after adaptation. Incubation in low concentrations of the isoflavones genistein and daidz...

Etiolated hypocotyls of the resistant soybean (Glycine max [L.] Merr.) cultivar Harosoy 63 became susceptible to Phytophthora megasperma (Drechs.) f.sp. glycinea (Hildeb.) Kuan and Erwin race 1 after treatment with abscisic acid. Susceptibility was expressed by increases in lesion size and a major decrease in accumulation of the isoflavonoid phytoalexin, glyceollin. In untreated hypocotyls, act...

Leguminosae, Papilionoideae, Diphysa, Isoflavonoids, Isoflavanones A m ajor phytoalexin produced by the fungus-inoculated leaflets o f Diphysa robinioides has been identified as the new isoflavonoid 5 ,7 ,2 ',4 '-te trahydroxy-6-(3 ,3-dim ethylally l)isoflavanone (diphysolone). It is accom panied by sm aller quan tities o f d iphyso lone-4 ' (or 2 ')-0 -m e th y l ether (diphysolidone), and the...

Structures of three antioxidant isoflavonoids isolated from the cultured broth of Streptomyces sp. OH-1049 were shown to be 4',7,8-trihydroxyisoflavone (1), 3',4',7-trihydroxyisoflavone (2) and 8-chloro-3',4',5,7-tetrahydroxyisoflavone (3), respectively. Among them, 3 is a novel isoflavonoid possessing a chlorine atom in the molecule. Compound 1 was synthesized and its antitumor activities were...

Astragalus membranaceus (Fisch.) Bunge has been was successfully acclimated in Central Europe. We report the content of isoflavones and some other polyphenolic compounds in roots and aerial parts that have been analyzed by means of TLC and HPLC. The total amount of isoflavones in leaves, was 0.55 mg g(-1) dry weight, and of the flavonols--up to 3.54 mg g(-1). In the roots isoflavonoid content w...

The optimization of a high-performance liquid chromatographic method to determine three isoflavonoids (daidzein, genistein, and biochanin A) in the fruit of Psoralea corylifolia is developed and validated. Dried psoralea fruit powder is extracted with aqueous methanol followed by the hydrolysis of the analytes' conjugated glycosides with hydrochloric acid. The HPLC assay is performed on a rever...

Isoflavon merupakan kelompok senyawa isoflavonoid dengan fungsi fisiologis yang bermanfaat bagi tubuh manusia. Bioaktivitas isoflavon berasal dari potensinya sebagai antioksidan dan fungsinya kesehatan seperti antikanker, antidiabetes, antiinflamasi. dalam bentuk tak terkonjugasi atau aglikon dianggap lebih aktif daripada glukosida. Sprouting salah satu metode pengolahan untuk meningkatkan bioa...