The induction and synthesis of metallothionein was studied in the isolated perfused rat liver, using Cd(I1) as the inducing metal. Perfusing the liver with 25 to 100 pg of Cd(I1) for 180 min revealed that the amount of Cd(I1) in metallothionein increased with the dosage of Cd(II), saturation being achieved at 75 pg. Zn(I1) in metallothionein decreased to about 60% of the level found in control ...

A series of experiments, performed at Johns Hopkins University, which led to our finding that plasticizers are extracted from poly-(vinyl chloride) (PVC) plastic by blood and biologic fluids will be described. The experiments began with the appearance of an unknown compound in the isolated perfused liver system. Livers were perfused with a mixture of synthetic plasma containing bovine serum alb...