BACKGROUND The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, and the ability to maintain transgene expr...

A 22 year-old lady with multi-drug-resistant pulmonary tuberculosis was on Kanamycin, Cycloserine, Ethionamide, Pyrazinamide and Moxifloxacin since more than two months. She presented with muscle cramps and carpopedal spasm. Investigation revealed hypokalemia and metabolic alkalosis. She also had hypomagnesemia, hypochloremia and hypocalciuria. Serum urea and creatinine levels were normal. Pati...

The production of kanamycin by three species of Streptonbyces and four fermentation media was studied. Streptomyces canus in medium No. 2 gave the highest yields of kanamycin aftrr 72 hrs. of fermentation. The presence of both molasses and glucose in the fermentation mcdium was an important factor for the production of kanamycin. The best concentration of molasses and glucose were 15.0 and 10.0...

OBJECTIVE To determine whether regenerated hair cells in the basilar papilla of chickens are resistant to kanamycin monosulfate damage. DESIGN Randomized controlled trial. SUBJECTS Ninety newly hatched Roman chickens. INTERVENTION Chickens were injected with kanamycin monosulfate (200 mg/kg/d) for 10, 13, 17, 20, 25, or 30 days. RESULTS Scanning electron microscopy revealed that hair ce...

Kanamycin and paromamine were inactivated in the presence of ATP and Mg++ by an enzyme solution obtained from E. coli carrying R factor. The inactivated kanamycin was isolated and it was found that the hydroxyl group on C-3 of 6-amino-6-deoxy-D-glucose moiety was phosphorylated. In the inactivated paromamine, the hydroxyl group on C-3 of D-glucosamine moiety was phosphorylated. Besides the inac...

The biochemical mechanism of resistance to kanamycin, viomycin, and rifampin in five clinical isolates of Mycobacterium tuberculosis was studied. Resistance to viomycin and kanamycin was attributed to altered ribosomes, whereas resistance to rifampin was attributed to an alteration of RNA polymerase. Ribosomal resistance was, however, not the only way of expressing resistance to viomycin and ka...

Effects of kanamycin and streptomycin on histamine release from rat mast cells were examined in response of the cells to concanavalin A(Con A) plus phosphatidylserine (PS), phytohemagglutinin (PHA) plus PS or a mixture of low-molecular-weight polymers of P-methoxy-N-methylphenethylamine (compound 48/80). In the response to each of the above stimuli, kanamycin (20 mM) or streptomycin (20 mM) cau...

OBJECTIVES Worldwide spread of a limited number of Panton-Valentine leucocidin (PVL) -producing methicillin-resistant Staphylococcus aureus (MRSA) clones has been reported in various communities. The objective of this study was to describe the molecular characteristics of the first PVL-positive MRSA strains isolated in Belgium. METHODS Clinical MRSA isolates (n = 41) collected from 2002 to 20...

We examined the molecular mechanisms of resistance to kanamycin and viomycin in Mycobacterium smegmatis. All of the M. smegmatis strains with high-level kanamycin resistance had a nucleotide substitution from A to G at position 1389 of the 16S rRNA gene (rrs). This position is equivalent to position 1408 of Escherichia coli, and mutation at this position is known to cause aminoglycoside resista...