نتایج جستجو برای: katg mutants
تعداد نتایج: 76510 فیلتر نتایج به سال:
The objective : to study prevalence and patterns of mutations in the katG, inhA, ahpC, rpoB genes associated with Mycobacterium tuberculosis (MTB) resistance isoniazid (H) rifampicin (R) patients various clinical manifestations pulmonary (TB). Subjects Methods . 441 sputum samples collected were tested using biological microchips. Tests carried out Group 1 - confirmed bacterial excretion (n = 2...
We studied the presence of mutations in the whole katG gene and specific regions of the oxyR-ahpC and mabA-inhA regulatory region in 61 Mycobacterium tuberculosis isoniazid-resistant isolates. An 81-bp region of the rpoB gene was also sequenced in 17 rifampin-resistant strains. Alterations in the katG gene were detected in 55% of the isolates. Mutation in codon 315 was the most prevalent (32%)....
OBJECTIVES Three Mycobacterium tuberculosis genetic loci--rpoB and katG genes and the fabG1(mabA)-inhA operon promoter region--were studied to reveal the mutations associated with rifampicin and isoniazid resistance. METHODS Four hundred and twelve isolates of M. tuberculosis from different regions of the Russian Federation were collected during 1997-2005. A matrix-assisted laser-desorption i...
چکیده زمینه و هدف: ایزونیازید یکی از داروهای مهم خط اول درمان سل میباشد. مقاومت به این دارو در بسیاری از نقاط جهان رو به افزایش است. جهشهای ایجاد شده در ژنKatG و inhA در اغلب موارد عامل مقاومت به ایزونیازید میباشند. هدف از این مطالعه ارائه روشی مناسب و سریع برای شناسایی موتاسیونهای مرتبط با مقاومت به ایزونیازید در مایکوباکتریوم توبرکلوزیس میباشد. روش بررسی: در این مطالعه وجود جهش در نوا...
Isoniazid (INH) and rifampin (RIF) are two of the most important antituberculosis drugs, and resistance to both of these drugs can often result in treatment failure and fatal clinical outcome. Resistance to these two first-line drugs is most often attributed to mutations in the katG, inhA, and rpoB genes. Historically, the identification and testing of the susceptibility of Mycobacterium tuberc...
Since the discovery of catalase, it has been postulated that aerobic organisms generate enough oxidants to threaten their own fitness and, in particular, their genetic stability. An alternative is that these enzymes exist to defend the cell against more-abundant oxidants imposed by external sources. These hypotheses were tested directly through study of Hpx- (katG katE ahpCF) mutants of Escheri...
Discovery of genotypic markers associated with increased transmissibility in Mycobacterium tuberculosis would represent an important step in advancing mycobacterial virulence studies. M. tuberculosis strains may be classified into one of three genotypes on the basis of the presence of specific nucleotide substitutions in codon 463 of the katG gene (katG-463) and codon 95 of the gyrA gene (gyrA-...
Ciprofloxacin is one of the most widely used antibiotics for the treatment of several infections caused by Gram-negative bacteria, like E. coli. Changes in gyrA, encoding GyrA subunit of DNA gyrase, cause the resistance to ciprofloxacin. Some ciprofloxacin resistant gyrA mutants acquired constitutive expression of marRAB operon due to the gaining mutations in marR, a repressor of this operon...
زمینه: تشخیص سریع و درمان دارویی مناسب اولین اقدام برای کنترل اپیدمی سل و سل مقاوم به دارو است. در مطالعه حاضر روش مولکولی pcr-rflp جهت تشخیص سریع سویه های کلینیکی و تعیین مقاومت آن به ایزونیازید مورد استفاده قرار گرفت. مواد و روش ها: در مطالعه حاضر تعداد 87 نمونه کلینیکی مایکوباکتریوم توبرکلوزیس مورد بررسی قرار گرفت. ابتدا با کمک pcr جستجو و تکثیر ژن katg قطعه bp620 برای تأیید مولکولی در تشخیص...
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