The maintenance of chlorophyll in darkened first leaves of oats was used as a bioassay for cytokinins in pea (Pisum sativum) roots. No cytokinin was found (in contrast with earlier reports on sunflower roots); however, the extracts contained two or more substances antagonistic to cytokinin, i. e., promoting the yellowing in this test. Because the most active of these appeared to be an amino aci...

background aloe vera l. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income. objectives in this study, the effect of different media on shoot proliferation of the shoot tip of aloe vera l. was investigated. materials and meth...

Tobacco mosaic virus (TMV) concentrations in tobacco callus tissues grown on the media containing cu-naphthaleneacetic acid (NAA) and kinetin at different concentrations were assayed periodically during successive cultures. Tobacco callus originally raised from the TMV-infected tobacco stem became green and compact when serially cultured on the medium containing lower concentration (0.01-o. 1 m...

A detailed study was done to understand the effect of phytohormones on morphology and growth performances of green filamentous algal taxa viz. Pithophora mooreana, P. cleveana, P. roettleri, Cladophora glomerata, Spirogyra orientalis, Oedogonium hindustanense, O. mexicanum, O. lemmermannii, O. rufescens f. minuta and Trentepohlia torulosa in vitro. Different proportion of 2, 4-Dichlorophenoxy a...

The defective splicing of pre-mRNA is a major cause of human disease. Exon skipping is a common result of splice mutations and has been reported in a wide variety of genetic disorders, yet the underlying mechanism is poorly understood. Often, such mutations are incompletely penetrant, and low levels of normal transcript and protein are maintained. Familial dysautonomia (FD) is caused by mutatio...

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were si...

Selected fluorescent dyes were tested for uptake by mitochrondria in intact cells of barley, maize, and onion. The cationic cyanine dye 3,3'-diheptyloxacarbocyanine iodide [DiOC(7)(3)] accumulated in mitochondria within 15 to 30 minutes without appreciable staining of other protoplasmic constituents. The number, shape, and movement of the fluorescent mitochondria could be seen readily, and the ...

Removal of deoxyribonucleic acid from meristematic onion root cells grown in solutions of kinetin, followed by metachromatic staining in azure B bromide, indicated the presence of appreciable amounts of ribonucleic acid in nuclei exposed to the cell division factor.

Farmers have been growing marigold commercially but they are still lacking knowledge on procedure of postharvest handling flowers. This has created a huge problem for those farmers since cannot store their own produce longer period time thus facing problems in marketing. In order to inform the with best means preservation cut flowers so that could fetch good market price product. A lab experime...