Mitochondrial dysfunction has been implicated in the pathophysiology of various cardiovascular diseases. CRIF1 is a protein present in the mitochondria associated with large mitoribosomal subunits, and CRIF1 knockdown induces mitochondrial dysfunction and promotes ROS production. p66shc is a redox enzyme implicated in mitochondrial ROS generation and translation of oxidative signals and, theref...

Colon cancer is the third most commonly diagnosed in men worldwide. grows slowly and metastasis has already occurred after diagnosis. Therefore, new targets are needed colon treatment Transmembrane proteins (TMEM) play a critical role presents different expression profile variety of tumor cells. TMEM106C TMEM family protein, but its on development unknown. In this study, we aimed to investigate...

Telomerase reverse transcriptase as the core component of telomere and most common non-coding region mutation gene in melanoma, may play a significant role development melanoma. The aim this study is to investigate anti-tumor effect human telomerase (hTERT) knockdown malignant melanoma its potential clinical value. We employed hTERT strategy detected expression target by transcriptase-quantitat...

The principal objective of this study was to evaluate the chemopreventive and therapeutic effects of a combination of all-trans-retinoic acid (RA) and knockdown of delta-like 1 homologue (Drosophila) (DLK1) on neuroblastoma, the most common malignant disease in children. As unfavorable neuroblastoma is poorly differentiated, neuroblastoma cell was induced differentiation by RA or DLK1 knockdown...

BACKGROUND YAP1, the nuclear effector of the Hippo pathway, has become an attractive target for treatment of malignancies and is a candidate oncogene in esophageal cancer (EC). We hypothesized that knockdown of YAP1 could suppress EC and could be used for targeted therapy. However, there are few reports of the effect of YAP1 knockdown in EC. MATERIALS AND METHODS Quantitative real-time polyme...

INTRODUCTION The 78-kDa glucose-regulated protein (GRP78) is induced in the cancer microenvironment and can be considered as a novel predictor of responsiveness to chemotherapy in many cancers. In this study, we found that intracellular reactive oxygen species (ROS) and nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation were higher in GRP78 knockdown DLD-1 colon cancer cel...

Chloride intracellular channel (CLIC) is a member of the chloride protein family for which growing evidence supports pivotal role in fundamental cellular events. However, physiological function CLIC insects still rarely uncovered. The ovary-derived High Five (Hi-5) cell line isolated from cabbage looper ( Trichoplusia ni ) widely used laboratories. Here, we studied both characteristics and func...

ABSTRACT Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and regulation of ZGA by maternal factors remains to be elucidated. In this study, depletion ring finger protein 114 (RNF114), ubiquitin E3 ligase, led developmental arrest two-cell mouse embryos. Using immunofluorescence transcriptome analysis, RNF114 was proven play crucial role major ZG...

Developmental Dyslexia is a reading disorder that affects individuals that possess otherwise normal intelligence. Until the four candidate dyslexia susceptibility genes were discovered, the cause of cortical malformations found in post mortem dyslexic brains was unclear. Normal brain development is crucial for the proper wiring of the neural circuitry that allow an individual to perform cogniti...

The oncogene c-Jun has been found to be up-regulated in a variety of cancers, including osteosarcoma. Doxorubicin is a frontline chemotherapeutic against osteosarcoma, but is limited by toxicity. DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. A biocompatible c-Jun DNAzyme nanoparticle formulated from chitosan regressed the growth and metastasis of pre-established tu...