BACKGROUND The mechanisms of virulence and species differences of Leishmania parasites are under the influence of gene expression regulations at posttranscriptional stages. In Iran, L. major and L. tropica are known as principal agents of cutaneous leishmaniasis, while L. infantum causes visceral leishmaniasis. METHODS As a preliminary study, we compared the proteome mapping of the above thre...

leishmania infantum is the most frequent cause of visceral leishmaniasis and l. tropica has been rarely linked to the disease in iran. in this study, bone marrow aspirates were collected from 10 child patients, suspected with visceral leishmaniasis referred to the pediatric wards of kerman medical hospitals, kerman, iran during 2002–2011. leishmania species were identified by using nested pcr i...

BACKGROUND Cutaneous leishmaniasis (CL) is a major public health problem in Libya. In this paper, we describe the eco-epidemiological parameters of CL during the armed conflict period from January 2011 till December 2012. Current spatiotemporal distributions of CL cases were explored and projected to the future using a correlative modelling approach. In addition the present results were compare...

During the past 20 years, cutaneous leishmaniasis has emerged as a major public health threat in Morocco. We describe distribution of Leishmania major and L. tropica in Morocco and a new focus of cutaneous leishmaniasis due to L. infantum. We recommend using molecular techniques to diagnose suspected leishmaniasis cases.

Leishmania tropica is one of the causative agents of cutaneous leishmaniasis (CL), a disfiguring parasitic disease that recently was found to be viscerotropic. In urban areas it is transmitted from infected individuals by the bite of phlebotomine sand flies to naïve persons (anthroponotic CL). In rural areas animals are thought to be the reservoir, but the full life cycle is still under investi...

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...

Human visceral leishmaniasis (VL) caused by L. infantum and cutaneous leishmaniasis (CL) caused by L. tropica and L. infantum have been reported in Turkey. L. infantum is also responsible for canine leishmaniasis (CanL) and it is widely common in the country. The main aim of the present study was to design a real-time PCR method based on the internal transcribed spacer 1 (ITS1) region in the di...

The infraspecific variability of the species Leishmania infantum is studied by using genetic markers generated by random amplified polymorphic DNA (RAPD). We have applied this technique, using 18 primers of arbitrary sequence, to 33 strains of the parasite belonging to 18 zymodemes isolated in different clinical forms and hosts. Other strains belonging to the species L. donovani, L. major, L. t...

a 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was exam­ined physically and microscopically for leishmaniasis. muco-cutaneous lesions containing a large num­ber of amastigotes of leishmania spp. were observed . amastigotes were also detected in liver and spleen of the puppy. the animal was positive with dipstick rk39 kit and high level of anti- leishmania anti...