The cardiac troponin C (cTnC) mutation, L29Q, has been found in a patient with familial hypertrophic cardiomyopathy. We previously showed that L29, together with neighboring residues, Asp2, Val28, and Gly30, plays an important role in determining the Ca(2+) affinity of site II, the regulatory site of mammalian cardiac troponin C (McTnC). Here we report on the Ca(2+) binding characteristics of L...

Lightfoot, Richard T., Steven Khov, and Harry Ischiropoulos. Transient injury to rat lung mitochondrial DNA after exposure to hyperoxia and inhaled nitric oxide. Am J Physiol Lung Cell Mol Physiol 286: L23–L29, 2004. First published February 7, 2003; 10.1152/ajplung.00352.2002.—The effect of hyperoxia alone and in combination with inhaled nitric oxide (NO) on the integrity of lung mitochondrial...

C100 emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the tunnel opening and forms a sheltered folding space. The 3.5Å crystal structure of the large ribosomal subunit of the eubacterium Deinococcus radiodurans, D50S, in complex with the TF binding domain (TFa) from Deinococcus radiodurans, reveals for the first time the molecular structure of the entire TFa bound...

There has been a long tradition of the interest of logicians in computer science and of computer scientists in logic. On the one hand, many problems raised and investigated by logicians before the emergence of computer science turned out to be relevant for the new science. We may quote here the notion of algorithm , model theory or relational systems. On the other hand, some problems stemming f...

The sites of phosphorylation of the interferon (IFN)induced protein designated Pi isolated from mouse L29 cells were characterized by partial proteolysis peptide mapping and by phosphoamino acid analysis. The Mr = 67,000 protein P1 phosphorylated in the presence of double-stranded RNA (dsRNA) was isolated from ribosomnal salt wash fractions of untreated and IFNtreated cells by poly(rI).poly(rC)...

Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI, which forms glutathione) and the glutathione S-transferase IsoILR1 from Rhod...