UNLABELLED Metabolic interactions with endosymbiotic photosynthetic dinoflagellate Symbiodinium spp. are fundamental to reef-building corals (Scleractinia) thriving in nutrient-poor tropical seas. Yet, detailed understanding at the single-cell level of nutrient assimilation, translocation, and utilization within this fundamental symbiosis is lacking. Using pulse-chase (15)N labeling and quantit...

The relationship of erythromycin biosynthesis to growth appears to vary with the fermentation medium in which the microorganism is grown. In a medium containing a complex nitrogen source, antibiotic production occurred only when growth was halted, whereas in a glycine-containing synthetic medium the two parameters increased at nearly the same time. An autostimulation of antibiotic production th...

Investigations with the isotopic tracer N16 have furnished definite evidence that ammonia is the key intermediate in nitrogen fixation by Azotobacter vinelundii (1). Direct isolation of the intermediate, however, has not been possible, apparently because ammonia is used so rapidly by this bacterium that it does not accumulate. In a study of the comparative biochemistry of nitrogen fixation, we ...

Objective Many livestock owners use the granular form of urea nitrogen during late summer and fall trying to increase forage growth for “stockpiled” forage. Livestock are then allowed to graze the “stockpile” at a later date when other forages no longer are growing or available. This practice extends the grazing season and reduces the need for higher priced stored feed. Urea is a common nitroge...

Studies in which (15)N-labeled precursors of urinary ammonia were infused into the artery of an intact functioning kidney of an acidotic dog have led to the following conclusions: Preformed ammonia and ammonia derived from the amide nitrogen of plasma glutamine are added directly to urine without significant incorporation into amino acid intermediates of renal tissue. Thus, reductive amination ...

Comparison of ion-molecular reactions of free-phenyl cations generated by tritium β -decay with 2-methyl- and 2-phenylquinolines has been investigated. The reaction of direct nitrogen atom phenylation with the help of nucleogenic phenyl cations has been fulfilled for the first time and a new one-step synthesis of tritium-labeled N-phenyl-2-phenylquinolinium salt-lipophilic radioactive biologica...