Establishing the analytical platforms for characterizing human milk oligosaccharides is important to fully assess their specific functionalities. The characterization of these biomolecules, however, is still considered challenging, owing to their overall complexity and diversity. Addressed here are the technical difficulties with an emphasis on the application of mass spectrometry to rapidly pr...

Our study investigated the potential for important in vivo drug-nutrient transport interactions at the lactating mammary gland using the L-carnitine transporter substrates, cefepime and L-carnitine, as proof-of-concept. On d 4 (n = 6/treatment) and d 10 (n = 6/treatment) of lactation, rats were administered cefepime (250 mg/h) or saline by continuous i.v. infusion (4 h). Serum and milk L-carnit...

The present study aimed to determine the potential of front face fluorescence spectroscopy (FFFS) to discriminate between milk samples belonging to Sicilo-Sarde ewes fed with three different feeding groups –named control, scotch bean meal and soybean meal– throughout the lactation stage (11 weeks). Milk samples were classified by factorial discriminant analysis. Similar results were obtained by...

The study aimed to determine the effect of different fixed factors (breed, region, calving year, season, parity and lactation stage) on daily milk production (yield content) four dairy cattle breeds under heat stress conditions (THI?72). research was conducted a set test-day records (Simmental, Holstein-Friesian, Red Holstein Brown Swiss) reared in Republic Serbia (Region Ma?va, Podunavlje Šuma...

Minerals and some biochemical parameters determined from blood serum analyses in left displacement abomasum (LDA) affected and healthy dairy cows to evaluate the effect of displacement abomasum on mineral status, energy metabolism and liver function. Samples were collected from 60 affected cows and 60 healthy control cows matched with cases, based on herd, parity, and stage of lactation. Concen...

The aim of this study was to compare the protein and fatty acid fractions of Balkan donkey and human milk in the early lactation stage (40 and 90 day). This study revealed that donkey milk contains αs1-casein (1.38-1.89 g/L) and higher concentration of β-casein (0.1-0.55 g/L) in comparison to human milk. The concentration of α-lactalbumin increased during the lactation phases from 40 to 90 days...

Characteristic of the udder, milk traits and their relationship were investigated in 32 Awassi ewes raised in a commercial flock. Commencing one month after lambing milk yield was recorded at monthly intervals until the ewes were dried. Also, the udder traits were measured, including udder width, udder circumference, udder length, distance between teats and length and diameter of both teats. Re...

The objective of this study was to estimate the genetic parameters of milk protein yields in Iranian Holstein dairy cattle. A total of 1,112,082 test-day milk protein yield records of 167,269 first lactation Holstein cows, calved from 1990 to 2010, were analyzed. Estimates of the variance components, heritability, and genetic correlations for milk protein yields were obtained using a random reg...

Maternal obesity and high-fat diet (HFD) predisposes offspring to obesity and metabolic diseases. Due to uncoupling, brown adipose tissue (BAT) dissipates energy via heat generation, mitigating obesity and diabetes. The lactation stage is a manageable period for improving the health of offspring of obese mothers, but the impact of maternal HFD during lactation on offspring BAT function is unkno...