Latex agglutination assays utilizing polyclonal antibodies were developed for the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups. Rabbit antisera were affinity purified through protein A/G columns, and the isolated immunoglobulins (IgGs) were covalently immobilized onto polystyrene latex particles. The resulting latex-IgG complex had a protein (IgG) load of 0.20 to 0...

BACKGROUND Sensitization to natural rubber latex has been linked to proteins from medical latex gloves. Various assays to estimate the amount of residual allergenic proteins extractable from latex gloves to assess their potential exposure hazard have inherent weaknesses. OBJECTIVE This investigation was aimed at developing 2-site immunoenzymetric assays and identifying appropriate protein mar...

Two-dimensional (2-D) electrophoresis followed by immunoblotting and N-terminal protein microsequencing were used to characterize and identify the IgE-reactive proteins of Hevea latex that are the main cause of the latex type I allergy affecting especially health care workers and spina bifida children. This approach generated a comprehensive latex allergen database, which facilitated the integr...

PURPOSE Instillation of sterile 1.0 microM latex beads into the central corneal epithelium renders Chinese hamsters resistant to corneal infection with Acanthamoeba castellanii. By contrast, activation of the adaptive immune response by subcutaneous immunization with A. castellanii antigens fails to protect against Acanthamoeba keratitis. This study was undertaken to examine the mechanisms that...

Latex allergy is recognized worldwide as a serious health risk. To date, exposure assessment and intervention strategies have focused primarily on respiratory protection; this work evaluates the potential role of dermal protein penetration in the development of latex allergy. In vitro penetration models using flow-through diffusion cells and both human surgical specimens and hairless guinea pig...

PURPOSE Children with spina bifida, bladder exstrophy and anorectal anomalies are at risk for latex allergy. Severe intraoperative anaphylaxis in a boy treated with kidney transplantation prompted this study to evaluate the prevalence of latex allergy in a cohort of children with chronic renal failure (CRF). MATERIALS AND METHODS Between 1996 and 2002, 57 boys and 28 girls were investigated a...

To improve the performance of concrete, polymers are mixed with concrete. It has been observed that polymer-modified concrete (PMC) is more durable than conventional concrete due to superior strength and high durability. In this research, effect of Styrene-Butadiene Rubber (SBR) latex on compressive strength and flexural strength of concrete has been studied and also the optimum polymer (SBR-La...

Accepted 20 November 1996 Abstract Objectives-To determine the prevalence of latex sensitisation among a large group of healthcare workers, study the occupational and non-occupational factors associated with latex allergy, and characterise latex exposure in air and by gloves. Methods-All 2062 employees of a general hospital in Hamilton, Ontario, Canada who regularly used latex gloves were invit...

Hypersensitivity reactions caused by latex products are ascribed to proteins elutingfrom the products, but the detailed properties of the responsible proteins have not been revealed yet. We have speculated that defense-related proteins biosynthesised in a rubber tree comprise the major part of the allergens. The aim of this study is to ascertain whether defense-related proteins are extracted fr...

BACKGROUND Allergy to latex has become an increasing concern to many patients and health care providers. Health care providers should become aware of this problem and develop strategies for treatment and prevention. METHODS We searched MEDLINE from 1990 to 1998 for the topics of latex allergy using the key words "latex" and "allergy." Other sources were found from back-referencing these refer...