PsSat5, a cDNA clone from Pisum sativum cv. Alaska, contained a microsatellite consisting of 15 TCA repeats within the 59UTR. This SSR microsatellite was immediately upstream of the presumptive ATG start codon. PCR amplification of genomic DNA from cv. Alaska yielded an identical sequence. This repeat region was analyzed from 10 additional wild and cultivated accessions of Pisum and from four c...

Symptoms similar to those produced by the phytoplasma disease "Big bud" were observed on tomatoes (Solanum lycopersicum L.) grown in field Mosul city, Iraq. Diseased plants characterized twisting, corrugated, yellowing or reddening of leaves. The sepals flowers acquired hypertrophied form, fused together and created a bell-shaped sterile bud (phyllody) green anthocyanin color. stems lignified, ...

Tomatoes (Lycopersicon esculentum) express two forms of leucine aminopeptidase (LAP-A and LAP-N) and two LAP-like proteins. The relatedness of LAP-N and LAP-A was determined using affinity-purified antibodies to four LAP-A protein domains. Antibodies to epitopes in the most N-terminal region were able to discriminate between LAP-A and LAP-N, whereas antibodies recognizing central and COOH-termi...

abstract lettuce is one of the best accumulators of heavy metals and nitrate between leafy vegetables. through this research, it was tried to decreased cadmium absorption with humic acid (ha) and studies the effect of ha on the accumulation and metabolism of nitrate. plants were grown in the peat/perlite and irrigated with hoagland solution with addition of cadmium (2 and 4 mg l -1) and ha (100...

Four AP1/AGL9 functional MADS box genes were characterized from the orchid (Oncidium Gower Ramsey). OMADS6 is a SEP3 ortholog, OMADS11 is a SEP1/2 ortholog, OMADS7 is an AGL6-like gene and OMADS10 is a putative paleoAP1 ortholog. The identity of these four genes was further supported by the presence of conserved motifs in the C-terminal regions of the proteins. OMADS6 showed an expression patte...

the influenza a virus is of global concern for the poultry industry, especially the h5 subtype as it has the potential to become highly pathogenic for poultry and mankind. recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. the goal of the present study was to investigate the possibility of expressing the ha1 protein in nicotiana tab...

The effect of different thiourea (TU) concentrations (10 and 20 mM) on antioxidants, protease activity and protein content with and without H2O2 (50 mM) induced stress was investigated in wheat leaves. A gradual decrease in protein content with a peak at 72 hours was observed in stressed as well as control leaves. This gradual decrease in leaf protein content at different time intervals was com...

In situ PCR is a technique that allows specific nucleic acid sequences to be detected in individual cells and tissues. In situ PCR and IS-RT-PCR are elegant techniques that can increase both sensitivity and throughput, but they are, at best, only semiquantitative; therefore, it is desirable first to ascertain the expression pattern by conventional means to establish the suitable conditions for ...

Transgenic tobacco (Nicotiana tabacum L, cv. SR-1) expressing mannitol 1-phosphate dehydrogenase, MTLD, in chloroplasts and myo-inositol O-methyltransferase, IMT1, in the cytosol after crossing of lines which expressed these foreign genes separately has been analysed. Plants expressing both enzymes accumulated mannitol and D-ononitol in amounts comparable to those following single gene transfer...