Fusion of the lentogenic strain "Clone 30" of Newcastle disease virus (NDV) with the cell line COS-7 has been studied. Fusion was monitored using the octadecylrhodamine B chloride dequenching assay [Hoekstra, D., de Boer, T., Klappe, K. and Wilschut, J. (1984). Biochemistry 23, 5675-5681]. In the present work, fusion of NDV with COS-7 cells was found to occur in a time- and temperature-dependen...

This study is aimed at evaluating antibody responses, viral loads, clearance and growth rate of Tanzanian free-range local chicken (FRLC) challenged with LaSota strain Newcastle disease virus (NDV) as indicator traits for selection chickens breeding enhanced resistance to the economic value. Three popular ecotypes: Kuchi, Ching’wekwe Morogoro-medium from three ecological zones Tanzania we...

despite routine vaccination programs against newcastle disease (nd), sporadic cases have occasionally occurred that remain a constant threat to commercial poultry. ten isolates of newcastle disease viruses (ndv) from infected broiler chicken cases were obtained from various locations in fars province during 2009-2011 and genetically analyzed using reverse transcription polymerase chain reaction...