An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to ...

Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated transduction occurs via fluid-phase macropinocytosis involving an intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagg...

2 Acknowledgements The completion of this master thesis would not have come about without the help and encouragement of several people. They should not be left unmen-tioned. First of all, I would like to thank Jan van den Berg, the supervisor of this thesis, for his guidance and for the many helpful suggestions and comments he made regarding the thesis. I am also grateful to Ludo Waltman, a ver...

The first part of this master project report is the literature review of the switched reluctance motor (SRM) which includes the basic principle of the switched reluctance motor, motor topologies, mathematical approach, torque production, electromagnetic, drives, converters and its applications. This part means to help to understand the working principle and properties of SRM. The second part of...

Acknowledgement I would like to thank some of the people who made this work possible, feasible and pleasurable. I would like to express my thanks to Karl Henrik Johansson, my supervisor at KTH, for his detailed review and constructive comments, and for having involved me in this interesting project. I wish to express my warm and sincere thanks to my home university supervisor Maria Domenica Di ...

Reshaping of beach nourishments under high angle of wave incidence i Preface The following report presents the Master of Science Thesis titled " Reshaping Mechanisms of Beach Nourishments under High Angle Wave Conditions " submitted as a partial fulfillment of the requirements for the degree of Master of Science (MSc) in Civil Engineering, the main supervision of Ir. Bas Huisman to whom I would...