The antimicrobial peptide nisin exerts its activity by a unique dual mechanism. It permeates the cell membranes of Gram-positive bacteria by binding to the cell wall precursor Lipid II and inhibits cell wall synthesis. Binding of nisin to Lipid II induces the formation of large nisin-Lipid II aggregates in the membrane of bacteria as well as in Lipid II-doped model membranes. Mechanistic detail...

Trehalose preserves lipid bilayers during dehydration and rehydration by replacing water to form hydrogen bonds between its own OH groups and lipid headgroups. We compare the lipid conformation and dynamics between trehalose-protected lyophilized membranes and hydrated membranes, to assess the suitability of the trehalose-containing membrane as a matrix for membrane protein structure determinat...

BACKGROUND Protein-lipid interactions play essential roles in the conformational stability and biological functions of membrane proteins. However, few of the previous computational studies have taken into account the atomic details of protein-lipid interactions explicitly. RESULTS To gain an insight into the molecular mechanisms of the recognition of lipid molecules by membrane proteins, we i...

We previously reported an approach for intracellular protein delivery by attenuating membrane-lytic activity of cationic amphiphilic peptides on cell surfaces. HAad is one such that cytosolically delivers proteins interest, including antibodies, stimulating their endosomal escape. Additionally, elicits ruffling membrane, accompanied transient membrane permeabilization, allowing the efficient cy...

The lipid A moiety of lipopolysaccharide forms the outer monolayer of the outer membrane of most gram-negative bacteria. Escherichia coli lipid A is synthesized on the cytoplasmic surface of the inner membrane by a conserved pathway of nine constitutive enzymes. Following attachment of the core oligosaccharide, nascent core-lipid A is flipped to the outer surface of the inner membrane by the AB...

This article evaluates the effect of membrane active channel-forming polyene antibiotic (PA) of levorin and its alkyl derivatives on the muscle performance. The membrane channels of muscle cells are capable to transport ions of potassium, sodium, and calcium. In the period of an intensive muscle exercise, the necessity for organic substrates increases and these channels start to work with the g...