Development of Methodology and Characterization of Ruminal Lipase-Producing Bacteria In Vitro. (May 2011) Holly Danielle Edwards, B.S., Iowa State University Co-Chairs of Advisory Committee: Dr. Rhonda K. Miller Dr. T. Matthew Taylor Hydrolysis of dietary lipids to free fatty acids (FFA) is a prerequisite for ruminal biohydrogenation, a bacterially mediated process that extensively saturates un...

A detailed investigation was undertaken to determine the effects of four single starter strains, Lactococcus lactis subsp. lactis 303, Lc. lactis subsp. cremoris HP, Lc. lactis subsp. cremoris AM2, and Lactobacillus helveticus DPC4571 on the proteolytic, lipolytic and sensory characteristics of Cheddar cheese. Cheeses produced using the highly autolytic starters 4571 and AM2 positively impacted...

An extract of ovine and bovine pineal glands (labeled Precipitate II) has previously been shown to possess lipolytic activity on rabbit adipose tissue. This extract also causes darkening of the frog’s skin in vivo and in vitro. Both lipolytic and melanotropic activities are abolished by trypsin and chymotrypsin. Fractionation of ovine pineal Precipitate II by the sequence, chromatography on die...

The aim of this study was to evaluate the effect of proteolytic (Serratia liquefaciens, match %: 99.39) or lipolytic (Acinetobacter genomospecies 10, match %: 99.90) psychrotrophic bacteria (bacterial counts, analysis of free fatty acids (FFA) and analysis of free amino acids) on the microbial and chemical properties (yogurt composition), and sodium dodecyl sulfate polyacrylamide gel electropho...

Five total mixed rations prepared from finger millet (Eleusine Coracana) straw as a roughage (48%) and mixed concentrate (52%), supplemented with a 1% isoacid mixture (i-C4, i-C5, C5 and phenylacetic acid in equal proportions) or oil (groundnut oil, 5% more than the control) or urea (5% more nitrogen than the control), and protein (groundnut cake, 5% more nitrogen than the control) were given i...

Ayeast isolate, selected for its lipolytic activity from a meat product, was characterized as Pichia anomala. Lipolytic activity, determined on p-NPA as esterase, was maximum at 28 ◦C, pH 6.5, and induced by the short chain triglyceride tributyrin. Fermentations in 2 L and 10 L stirred tank bioreactors, with 20 and 60 g/L glucose respectively, showed that in the second case lipolytic activity i...

The intravascular injection of heparin results in the rapid appearance in the blood of a lipolytic enzyme capable of clearing lipemic sera and hydrolyzing artificial triglyceride emulsions (1, 2). This enzyme has been termed clearing factor (3), lipoprotein lipase (4), clearing factor lipase (2), or postheparin plasma lipolytic activity (PHLA) (5). A lipolytic enzyme with characteristics nearly...

This study was aimed at evaluating the capability of Yarrowia lipolytica W29 for the synthesis of lipolytic enzymes in a medium containing plant oils from non-conventional sources with some components displaying bioactivity. Oils from almond, hazelnut, and coriander seeds were obtained by using n-hexane (Soxhlet method) and a chloroform/methanol mixture of solvents (Folch method), and their eff...

The present study was undertaken to determine the relationship between the antilipolytic and lipolytic effects of insulin on hormone-stimulated lipolysis and the mechanisms of these reactions. The dose-response curve of norepinephrine-stimulated lipolysis in rat adipocytes was not sigmoidal but biphasic in nature. Intracellular free fatty acid levels were linearly related to lipolytic rate and ...