Rat liver microsome fractions contain two general kinds of inhibitors of amino acid incorporation in vitro. The first is derived from lysosomes contaminating the microsome fractions. Lysosomes may be removed from rough vesicles (ribosomes plus endoplasmic membranes) by a density centrifugation technique. Lysosomal inhibition of incorporation in vitro is much more prominent in normal than in reg...

objectives: a wide range of liver and kidney disorders are associated with diabetes and there is a mutual relationship between diabetes and these diseases. herbal medicine with having abundant ingredients is one of these options. the goal of this study was to compare the effects of alcoholic extract of aerial parts of salvia hydrangea with glibenclamide on functional tests of liver and kidney i...

objective(s) quinazolinones are heterocyclic compounds, with biological and pharmacological activities, such as inhibiting some proteins, enzymes and reducing blood lipids. materials and methods following previous results of our group, effects of two new derivatives of quinazolinones 9(3)-quinazolinone-2-propyl-2-phenylethyl (qppe) and 9(3)-quinazolinone-2-ethyl-2-phenylethyl (qepe) on livers, ...

conclusions in general, it can be said that the application of nanoliposomal paromomycin sulfate formulations for topical treatment of the cutaneous leishmaniasis does not create serious side effects in the short term, but its long-term use leads to mild renal and liver side effects that requires more attention. background hepatotoxicity due to drugs is the most common cause of deaths. nephroto...

The role of nucleotides as precursors of ribonucleic acid in animal tissues has been well documented (l-4). ilside from the formation of polymers by the enzyme polynucleotide phosphorylase (5), only one preliminary report of net synthesis of polyribonucleotides in cell-free systems has been made (6). Hilmoe and Heppel (7) have demonstrated the phosphorolysis of added polyadenylic acid by a part...

Rapidly proliferating normal and malignant cells had a greater initial rate of amino acid incorporation into microsomal proteins than did normal cells. The rate of micro.somal protein renewal in Novikoff hepatoma was greater than that of liver cells. The initial rates of amino acid incorporation into hepatoma and liver ribonucleoprotein particles were approximately equal. The soluble fractions ...

The cytochrome P-450 enzyme which catalyses 25-hydroxylation of vitamin D3 (cytochrome P-450(25] from pig kidney microsomes [Postlind & Wikvall (1988) Biochem. J. 253, 549-552] has been further purified. The specific content of cytochrome P-450 was 15.0 nmol.mg of protein-1, and the protein showed a single spot with an apparent isoelectric point of 7.4 and an Mr of 50,500 upon two-dimensional i...

Isolated rat hepatocytes were used to determine the cytotoxicity of peripheral blood mononuclear cells (PBM) from patients with alcoholic liver disease (ALD) and chronic active hepatitis (CAH). The specificity of the cytotoxic effect on liver cells was monitored using rat kidney cells. PBM from patients with CAH and ALD showed increased spontaneous cell-mediated cytolysis (SCMC) for hepatocytes...