Four carbohydrate derivatives of poly-D-lysine have been synthesized and assayed as substrates for the tissue culture of embryonic mouse cerebellar cells. On poly-beta-(D-glucopyranosyl)-poly-D-lysine and on poly-beta-(N-acetyl-D-glucosaminyl)-poly-D-lysine, dissociated cerebellar cells formed a monolayer. On poly-beta-(D-galactopyranosyl)-poly-D-lysine, cellular aggregates were formed and cabl...

In this study, chitosan (Chit) supported titanium (Ti) nanoparticles (Ti@Chit NPs) were synthesized by chemical method. Ti@Chit NPs calculated to be 7.275 ± 2.15 nm and 18.629 according scanning electron microscopy (SEM) atomic force (AFM) results, respectively. The modified the electrode a sensitivity study was carried out against lysine amino acid. It has been determined that catalytic perfor...

Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result...

We found that the simple addition of L-methionine to the wild type of Corynebacterium glutamicum results in excretion of the cellular building block L-lysine up to rates of 2.5 nmol/min/mg (dry weight). Biochemical analyses revealed that L-methionine represses the homoserine dehydrogenase activity and reduces the intracellular L-threonine level from 7 to less than 2 mM. Since L-lysine synthesis...

Two experiments were conducted to determine the influence of 15.6 and 29.4 degrees C. environmental temperatures on 2- to 4-week broiler lysine requirements. Weights for 4-week-old cockerels were maximum when either 1.10% dietary lysine in the 15.6 degrees C. environment or 1.00% dietary lysine in the 29.4 degrees C. environment were fed. Feed was used more efficiently by 4-week old cockerels f...

Two 42-d experiments were conducted with 60 and 100 Large White growing pigs, initially weighing 17.7 and 18.3 kg, respectively, to determine their ability to select between diets varying in lysine content. Dietary lysine levels ranged from .60 to 1.40%. A common basal diet (17% crude protein, 3.3 Mcal digestible energy/kg) based on corn, soybean meal and peanut meal was used in both experiment...

abstract this study aimed to investigate the effects of different levels of the amino acid lysine on growth performance, feed efficiency and body composition of juvenile snow trout (schizothorax zarudnyi). for this purpose, 150 fish with average weight 22.51±0.28 g fed with five levels of dietary amino acid lysine including 0 (control), 7, 12, 17 and 22 g/kg diet, for 8 weeks. fish fed two time...

enzymatic hydrolysis of yellowfin tuna visceral protein with neutrase has been carried out by response surface methodology using factorial design. peptide chain length was estimated as the response surface to the hydrolysis conditions (enzyme activity, reaction temperature, and reaction time). the tuna visceral protein hydrolysate had relatively high protein (74.56%) and low lipid content (1.86...

Lysine or Mg2+ alone at high concentrations brings about partial dimerization of the lysine-sensitive aspartokinase of Escherichia coli B. The native enzyme, which has a molecular weight of 75,300 and consists of 2 subunits, responds to a combination of lysine and Mg 2+ at much lower concentrations by dimerizing to a tetrameric molecule with a molecular weight approaching 150,000. This dimeriza...