The study of configurational parameters of deformed DNA is a relevant problem in research of such important biological process as double helix compactization in cell. The deformations accompanied with local disruptions of the regular macromolecule structure cause significant bending of the double helix, or kinks. In this paper an approach for Kratky-Porod model to calculate persistence length o...

Diffusional permeability (P) to inulin (P(in)), albumin (P(alb)), and dextrans [70 (P(dx 70)), 150 (P(dx 150)), 550 (P(dx 550)), and 2, 000 (P(dx 2,000))] was determined in specimens of parietal pericardium of rabbits, which may be obtained with less damage than pleura. P(in), P(alb), P(dx 70), P(dx 150), P(dx 550), and P(dx 2, 000) were 0.51 +/- 0.06 (SE), 0.18 +/- 0.03, 0.097 +/- 0.021, 0. 04...

The mechanisms underlying the pathological changes in lymphedematous tissue are far from clear, and it is becoming apparent that plasma proteins may not be the only key factors responsible for holding water in the interstitium. This review focuses on an "old" macromolecule--hyaluronan (HA) which is one of the major components of the interstitium and has a close relationship with the lymphatic s...

This study concerns parameterization and subsequent subtraction of the fast decaying signals of macromolecules from in vivo MRS signals of tissue metabolites of interest. The parameterization is done with a State Space approach (HSVD) based on singular value decomposition. The method is tested with a simulated non-exponentially damped macromolecule signal and an exponentially damped metabolite ...

The monomer analog of a common water-soluble charged macromolecule shows surface tilt and cooperative adsorption that would be expected for larger surfactant molecules—the small organic ion behaves as an embryonic surfactant. Yet the organic ion competes with inorganic ions (Na, Mg, etc.) for access to the surface, and therefore its tilt increases with the size of the competing coion. Similar o...

We derive a thermodynamic identity that allows one to infer the change in the number of screening ions that are associated with a charged macromolecule as the macromolecule is continuously stretched. Applying this identity to force-extension data on both single-stranded and double-stranded DNA, we find that the number of polymer-associated ions depends nontrivially on both the bulk salt concent...

Proteoglycan was isolated from cartilage and freed from contaminating glycoproteins and hyaluronic acid. The macromolecule consists of a protein core covalently linked to a number of glycosaminoglycan side chains, namely chondroitin sulphate and keratan sulphate. This proteoglycan retards the attachment of a variety of cell types to tissue culture plastic and to collagen. Glycosaminoglycans alo...

Introduction Prior knowledge of the macromolecule baseline is important for the accurate quantification of metabolite concentrations from in vivo H NMR spectra acquired at short echo times (TE) [1]. Macromolecules may be altered due to pathology [2]. In contrast, regional alterations of macromolecule baseline in rat brain have not been studied, although the neurochemical profile varies strongly...