The rice blast fungus, Magnaporthe oryzae, infects many economically important cereal crops, particularly rice. It has emerged as an important model organism for studying the growth, development, and pathogenesis of filamentous fungi. RabGTPases are important molecular switches in regulation of intracellular membrane trafficking in all eukaryotes. MoRab5A and MoRab5B are Rab5 homologs in M. ory...

The heterokaryotic and vegetative diploid phases of Magnaporthe grisea, a fungal pathogen of grasses, have been characterized. Prototrophic heterokaryons form when complementary auxotrophs are paired on minimal medium. Hyphal tip cells and conidia (vegetative spores) taken from these heterokaryons are auxotrophs with phenotypes identical to one or the other of the parents. M. grisea heterokaryo...

The subcellular expression patterns and fluorescence intensities of cytoplasm-targeted, constitutively expressed blue-, cyano-, green-, yellow- and red-fluorescent protein were assessed in a number of transformants of the blast pathogen, Magnaporthe grisea. All transformants grew normally, remained pathogenic on barley, and, except for those expressing blue fluorescent protein, exhibited signif...

Magnaporthe oryzae (M. oryzae) is a fungal pathogen and the causal agent of rice blast disease. Previous lipidomics analysis of M. oryzae demonstrated that trehalose, a carbohydrate common to various fungi and algae, is thought to be involved in the possible conversion of glycogen into triacylglycerides for energy, an important step in the pathogenesis of M. oryzae. A key enzyme responsible for...

BACKGROUND In our previous studies, we discovered a series of chalcone-based phytopathogenic fungus inhibitors. However, knowledge of their effects, detailed targets and molecular mechanisms in Magnaporthe oryzae (M. oryzae) remained limited. METHODS To explore the expression and function of differentially expressed genes in M. oryzae after treatment with compound C1, we analyzed the expressi...

Wheat blast (Magnaporthe oryzae Triticum) in Bangladesh and South America is recognized as one major limiting factor of wheat production. Its control using chemical pesticides raises concerns about food safety pesticide resistance, which have dictated the need for alternative management approach, nutrient supplementation could be an ecofriendly alternative. Experiments were carried out under co...

Screening for microorganisms with antagonistic activity against crop pathogens is essential microbial antagonist preparation, disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method screening endophytic bacteria displaying Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that ...

Marcus Chibucos (Virginia Bioinformatics Institute) [Phytophthora sojae and Phytophthora ramorum] Candace Collmer (Wells College) [Pseudomonas syringae] Jeremy D. Glasner (University of Wisconsin) [Erwinia crysanthemi] Michelle Gwinn Giglio (TIGRWorkshop organizer) [Prokaryote] Linda Hannick (TIGR) [Eukaryote] Rays Jiang (Virginia Bioinformatics Institute) [Phytophthora sojae and Phytophthora r...