the nutritional requirements of mammalian embryos changes throughout pre-implantation period, coincident with changes in the secretion of the female reproductive tract. therefore, it has been suggested that sequential culture media may support nutritional requirements for optimal growth of the mammalian embryos. in this study, we investigated the effect of two different concentrations (10 or 10...

Title of Document: EVALUATION OF LEF1 TRANSCRIPTION FACTOR IN MAMMALIAN PREIMPLANTAITON EMBRYOS Ashley Elaine Meece, Master of Science, 2007 Directed By: Associate Professor, Dr. Carol L. Keefer Department of Animal and Avian Sciences The first lineage differentiation of cells during pre-implantation embryo development is critical for continued embryonic growth. Though several transcription fac...

The permeability of the plasma membrane to water and cryoprotectants is one of the important factors for determining the suitable condition for the vitrification of mammalian oocytes and embryos. Water and cryoprotectants move slowly through oocytes and early embryos, principally by simple diffusion, in the mouse, bovine, pig, and human. In contrast, water, glycerol, and ethylene glycerol move ...

The permeability of the plasma membrane to water and cryoprotectants is one of the most important factors for determining suitable conditions for vitrification of mammalian oocytes and embryos. In mouse oocytes and early stage embryos, water and cryoprotectants move slowly, principally by simple diffusion. In contrast, in morulae (and probably blastocysts), water, glycerol, and ethylene glycero...

Chromatin remodelling proteins are essential for different aspects of metazoan biology, yet functional details of why these proteins are important are lacking. Although it is possible to describe the biochemistry of how they remodel chromatin, their chromatin-binding profiles in cell lines, and gene expression changes upon loss of a given protein, in very few cases can this easily translate int...

The first lineage allocation during mouse development results in the trophectoderm and inner cell mass at the blastocyst stage. The caudal-related transcription factor, CDX2, is upregulated and required for the repression of inner cell mass genes Oct 4 and Nanog in the trophectoderm to specify the two lineages. Mouse embryos are precocious implanters requiring early establishment of trophectode...

The goal was to gain understanding of how 12 genes containing SNP previously related to embryo competence to become a blastocyst (BRINP3, C1QB, HSPA1L, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3 and WBP1) participate in embryonic development. Gene expression was evaluated in matured oocytes and embryos. BRINP3 and C1QB were not detected at any stage. For most other genes, transcrip...

The first lineage allocation during mouse development results in the trophectoderm and inner cell mass at the blastocyst stage. The caudal-related transcription factor, CDX2, is upregulated and required for the repression of inner cell mass genes Oct 4 and Nanog in the trophectoderm to specify the two lineages. Mouse embryos are precocious implanters requiring early establishment of trophectode...

In vitro cultivation of early mammalian embryos under controlled con ditions in various kinds of media have been reported by numerous investigators. According to Austin's detailed review in 1961, the majority of studies in this field have involved the rabbit, mouse, and a few other mammalian species. It has been shown that rabbit ova will cleave readily and develop into morulae in a defined med...

Polarity formation in mammalian preimplantation embryos has long been a subject of controversy. Mammalian embryos are highly regulative, which has led to the conclusion that polarity specification does not exist until the blastocyst stage; however, some recent reports have now suggested polarity predetermination in the egg. Our recent time-lapse recordings have demonstrated that the first cleav...