In the biopharmaceutical industry, mammalian cell culture systems, especially Chinese hamster ovary (CHO) cells, are predominantly used for the production of therapeutic glycoproteins. Glycosylation is a critical protein quality attribute that can modulate the efficacy of a commercial therapeutic glycoprotein. Obtaining a consistent glycoform profile in production is desired due to regulatory c...

Near-infrared, Raman, fluorescence and X-ray fluorescence spectra of multiple soy hydrolysate lots manufactured by different vendors were analyzed for comprehensive characterization of raw materials used in mammalian cell culture processes. The variability of soy hydrolysates, as well as the correlation between multiple spectra and cell culture performance was addressed. The identified composit...

JUNE 2008 B ioreactor technologies for mammalian cell culture may seem quite sophisticated (1). In fact, efficient mammalian cell culture is simple and requires just two major elements: mixing and oxygen (O2) transfer. Traditional methods for mixing use an impellor, and the classical O2 transfer method features a sparging stone that expels air bubbles to increase the O2 transfer surface area in...

Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development work is done in laboratory bioreactors, in which carbon dioxide levels are more easily controlled. Some challenges in carbon dioxide control can p...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

statement of problem: many studies have indicated that genetic disturbances are common findings in patients with oral squamous cell carcinoma (oscc). identification of these changes can be helpful in diagnostic procedures of these tumors. purpose: the aim of this study was to appraise the chromosomal disorders in blood and tissue patients with oscc. methods and materials: in this descriptive st...

A ribozyme based gene control element enabled the spatio-temporal regulation of gene function in mammalian cell culture with light.

Abstract We report laser emission from gallium nitride (GaN) microrods that are introduced into mammalian cells and the application of these for cell labeling. GaN were grown on graphene-coated SiO 2 /Si substrates by metal-organic vapor phase epitaxy. The easily detached because weakness van der Waals forces between graphene. uptake HeLa via endocytosis viability after investigated. Normal cel...

In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic developmental competence. It is well-known that higher than physiological levels of ROS triggers granulosa ...