The fungal cell wall is the first point of interaction between an invading fungal pathogen and the host immune system. The outer layer of the cell wall is comprised of GPI anchored proteins, which are post-translationally modified by both N- and O-linked glycans. These glycans are important pathogen associated molecular patterns (PAMPs) recognised by the innate immune system. Glycan synthesis i...

Macrophages of the reticuloendothelial system and brain act as major reservoir for HIV because of their long term survival after HIV infection and ability to spread virus particles to bystander CD4 positive lymphocyte cells. The objective of the present study was to investigate mannan-coated nanoparticles for macrophage targeting of didanosine. Different didanosine loaded nanoparticles were pre...

Serum samples from 197 patients with and without candidiasis were assayed for Candida albicans mannan and Candida krusei mannan by an enzyme-linked immunosorbent assay (ELISA) and a latex agglutination test (LA) and for D-arabinitol by the enzymatic fluorometric method. Of the 43 patients positive for C. albicans mannan (> or = 0.2 ng/ml), 34 were infected with C. albicans and 9 were infected w...

Soybean meal is the most commonly used protein source in animal feeds. Among the undesirable attributes of soybean meal is the high level of β-mannan, which was determined to be detrimental to the growth performance of animals. β-Mannan is a type of hemicellulose in the plant cell wall and can be hydrolyzed by endo-β-mannanase. The goal of this study is to isolate and characterize an endo-β-man...

Two new chiral sandwich-type bis(phthalocyaninato) yttrium double-decker complexes including the homoleptic species (R)- and (S)-Y[Pc(OBNP)4]2 (1) and a heteroleptic analogue (R)- and (S)-Y(Pc)[Pc(OBNP)4] (2) {Pc = unsubstituted phthalocyaninate; [Pc(OBNP)4] = tetrakis(dinaphtho[1,2-e:1',2'-g]-1,4-(dioxocine)[2,3-b;2',3'-k;2'',3''-t;2''',3'''-c']phthalocyaninate)} have been synthesized and spec...

Background: Gradually, fungal infections are growing and have become a medical concern. Candida species one of the most common pathogens in immunocompromised patients, such as those with lung cancer causing invasive disease. Early diagnosis candidiasis is critical for patient care patients. Anti-mannan IgM IgG biomarkers used to diagnose candidiasis. This study aims determine relationship betwe...

To develop a general model of polysaccharide-peptide vaccine, we have investigated the efficiency of linear peptides derived from protein SR, and adhesin of the I/II protein antigen family of oral streptococci, to act as carriers for two T cell-independent polysaccharides: serogroup f polysaccharide from Streptococcus mutans OMZ 175 (poly f) and Saccharomyces cerevisiae mannan. Peptide 3 (YEKEP...

[Letter]. Lancet 1998;352:959–60. 3. Madsen HO, Garred P, Thiel S, Kurtzhals J, Lamm LU, Ryder LP, et al A. Interplay between promoter and structural gene variants control basal serum level of mannan-binding protein. J Immunol 1995;155:3013–20. 4. Madsen HO, Satz ML, Hogh B, Svejgaard A, Garred P. Different molecular events result in low protein levels of mannan-binding lectin in populations fr...

How simple monosaccharides, once polymerized, become the basis for structural materials remains a mystery. A framework is developed to investigate the role of water in the emergence of dynamic structure in polysaccharides, using the important beta(1-->4) linkage as an example. This linkage is studied within decasaccharide fragments of cellulose, chitin, mannan, xylan, and hyaluronan, using mole...

A lectin that displays specificity for the core region of asparagine-linked oligosaccharides (Man3GlcNAc2-Asn) was isolated from human serum and liver by affinity chromatography on mannan-Sepharose. The designation 'core-specific lectin' (CSL) is used to indicate its specificity. Selective elution of human CSL from mannan-Sepharose was accomplished with 50 mM-mannose. Two additional proteins th...