Characterizing the behavior and robustness of enzymatic networks with numerous variables and unknown parameter values is a major challenge in biology, especially when some enzymes have counter-intuitive properties or switch-like behavior between activation and inhibition. In this paper, we propose new methodological and tool-supported contributions, based on the intuitive formalism of temporal ...

Membrane-type 1 matrIx metalloproteinasethat is associatedwith the proteolytic activation of progelatlnase A was expressed as a recombinant fusion protein in Escherichia coiL The recombinant enzyme cleaved the propeptide sequence of gelatinase A in a sequence-specific manner. A mutant progelatinase A that has a substitution ofAsnss@Leu to ile-Val was not processed at all. The processing was blo...

Resin- dentin interface hydrolysis is one of the greatest problems in restorative dentistry, Because of collagenolytic and proteolytic activity of dentin enzymes. The aim of this study was to compare the effect of CHX 2% and NaOCl 5% as rewetting agent on resin-dentin micro tensile bond strength.Methods: 45 extracted, human caries-free third molar teeth were collected. After exposing of superfi...

In a series of 12 patients with inoperable gastric carcinoma who had treatment with a synthetic matrix metalloproteinase inhibitor (Marimastat) for more than one month, six developed a frozen shoulder or a condition resembling Dupuytren's disease. This suggests that the matrix metalloproteinases, a family of naturally occurring proteinases, may be involved in the pathogenesis of these two condi...