background: ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. in the current study, the purpose was to evaluate the effects of ammuniom chloride (ac) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of bcl-2. methods: in vitro maturation (ivm) was performed in t...

dendritic cells (dcs) induce pathogen-specific t cell responses. we comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), pha activated t cells (t cell conditioned medium), and mixture of fibroblast & pha activated t cells (fcm-tccm) conditioned media on maturation of dcs. monocytes were cultured with gm-csf and il-4 for five da...

Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...

Background: Cryopreservation of ovaries is an important technique in assisted reproduction technology. Physical forces like hydrostatic pressure have a pivotal role in reproduction systems. Due to changes in intrafollicular pressure during ovulatory process, this study designed to examine the effects of hydrostatic pressure on oocyte maturation and cell death in cumulus cells from cryopreserved...

The microbiology of Kasseri cheese, a Protected Designation Origin (PDO) cheese pasta-filata type was studied in order to identify the dominant species and strains that may contribute maturation process. Chemical composition microbiological quality samples from two different dairies during at 0, 7, 25, 60 90 days maturation. Lactic acid bacteria Enterococcus spp. were found be microflora fresh ...

Immature neurons undergo morphological and physiological maturation in order to establish neuronal networks. During maturation, a large number of genes change their transcriptional levels, these changes may be mediated by chromatin modifiers. In this study, we found that the level Ezh1, component Polycomb repressive complex 2 (PRC2), increases during mouse neocortical culture. addition, conditi...