A radioimmunoassay system has been developed for the measurement of two major metabolites of 9,3"-diacetylmidecamycin, Mb-6 and Mb-12. A radioimmunoassay for Mb-6 was performed by using anti-Mb-6 serum and a [125I]tyramined Mb-6 derivative as a radiolabeled antigen. The labeled antigen was prepared by the chloramine T method. The antiserum was obtained from a rabbit immunized with Mb-6 conjugat...

We used a combined immunochemical and molecular approach to ascertain the presence and concentrations of both the intracellular oxygen-binding hemoprotein myoglobin (Mb) and its messenger RNA (mRNA) in 13 of 15 known species of Antarctic channichthyid icefishes. Mb protein is present in the hearts of eight species of icefishes: Chionodraco rastrospinosus, Chionodraco hamatus, Chionodraco myersi...

Background & Aims of the Study: Methylene blue (MB) is a risk for human and environment. Adsorption process is one of the removal mechanisms of MB. The purpose of this research was the evaluation of low cost adsorbent of walnut bark granule for MB dye removal from aqueous environments. Materials & Methods: In this experimental research, the effect of various operating parameters...

In this work, orange (Citrus sinensis) peels biochar (OPBC) were prepared by one-step H2SO4 activation for Methylene Blue (MB) adsorption from aqueous solution. The physicochemical properties of OPBC were characterized using instrumental analyses such as CHNS-O analyzer, Fourier Transform InfraRed (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), X-Ray Diffract...

A novel metal–organic framework (MOF), with the formula [Cu(II)L]n (L=4, 4′-diamino diphenyl sulfone), has been synthesized conventionally andhydrothermally methods and characterized by FT-IR, PXRD, EDX, and SEMtechniques. The results MOFs were applied for photodegradation of MethyleneBlue (MB). The influence of affecting variables, such as initial MB dyeconcentrat...

GLUT1, the most ubiquitously expressed member of the GLUT family of glucose transporters, can be acutely activated by a variety of cell stresses. Methylene blue activates glucose transport activity of GLUT1 in L929 fibroblast cells presumably by a redox cycling of MB, which generates an oxidative stress. Data shown here reveal that methyl-beta-cyclodextrin (MCD) blocks both the staining of cell...

Myoglobin (Mb) catalyzes various two-electron oxidations; however, ferryl porphyrin cation radical equivalent to peroxidase compound I has not been identified yet. Distal histidine mutants of sperm whale Mb (His-64 --> Ala, Ser, and Leu) afford an apparent intermediate followed by the formation of a ferryl heme (Mb-II) in the reaction with m-chloroperbenzoic acid. Because the intermediate exhib...