A method for the isolation of lymphocyte plasma mem branes from patients with chronic lymphocytic leukemia is described. Lymphocytes were disrupted in a hypotonie bicarbonate medium using a Dounce homogenizer. The plasma membranes fraction was finally collected from a continuous sucrose gradient at density 1.115 (g/ml). Elec tron micrographs of this material showed membranes and small dense ves...

The interactions with water and the physical properties of microfibrillated celluloses (MFCs) and associated films generated from wood pulps of different yields (containing extractives, lignin, and hemicelluloses) have been investigated. MFCs were produced by combining mechanical refining and a high pressure treatment using a homogenizer. The produced MFCs were characterized by morphology analy...

l-Methionine γ-lyase (MGL), a pyridoxal 5'-phosphate-dependent enzyme, possesses anti-tumor activity. However, the low activity of MGL blocks the anti-tumor effect. This study describes an efficient production process for the recombinant MGL (rMGL) from Idiomarina constructed using the overexpression plasmid in Escherichia coli BL21 (DE3), purification, and large-scale production. The enzyme pr...

We have proposed a fruitful design principle targeting concentration ratio (CR) >1000× for typical high concentrating photovoltaics (HCPV) system, on account of two-concentrator system + homogenizer. The primary dual-lens concentrator unit, completely analogous basic optics seen in the superposition compound eyes, is trend not hitherto reported solar concentrators to our knowledge. Such cons...

the effect of mixing on rheological behavior of 6% wt epoxy-clay nanocomposites was studied. the mixing processes were carried out by low shear mixer, homogenizer and ultrasonic and combination of different mixing techniques at medium and maximum power. all these methods led to intercalated structure. the xrd results showed that the ultrasonic has the best effect on dispersion while a low shear...

The aim of this study is the development of nanoemulsions for intravenous administration of Sorafenib, which is a poorly soluble drug with no parenteral treatment. The formulation was prepared by a high energy emulsification method and optimized by response surface methodology. The effects of overhead stirring time, high shear rate, high shear time, and cycles of high-pressure homogenizer were ...

Through anther and microspore culture methods, complete homozygous plants can be produced within a year as compared to the long inbreeding method. Isolated microspore culture is one of the most important techniques for rapid development of haploid plants. The efficiency of this method is influenced by several factors such as cultural conditions, growth regulators, plant media, pretreatments, ph...