The herpes simplex virus type 1 (HSV-1) immediate early protein, ICP4, participates in the regulation of viral gene expression by both activating and repressing RNA polII transcription. We used affinity purification of ICP4 expressed in infected cells followed by mass spectrometry and western blot analysis to determine the composition of cellular complexes associated with ICP4 throughout infect...

Current mediator and wrapper architectures do not have the flexibility to scale to multiple wrapped sources, where some sources may be redundant, and some sources may provide incomplete answers to a query. We propose a metawrapper component which is capable of handling multiple wrapped sources, in a particular domain, where the multiple sources provide related information. The metawrapper makes...

Mediator recently has emerged as a central player in the direct transduction of signals from transcription factors to the general transcriptional machinery. In the case of nuclear receptors, in vitro studies have shown that the transcriptional coactivator function of the Mediator involves direct ligand-dependent interactions of the MED1 subunit, through its two classical LxxLL motifs, with the ...

The study explored the impact of optimism, resilience and religiosity on mental well-being perceived health as well their role in stress relation professions’ students. was carried out online with a total 123 students medicine, nursing, assistant-pharmacists social workers. Analyses suggest that optimism were associated appraisal less intensive, threatening more controllable. Religiosity not st...

The affordability of next generation sequencing (NGS) is transforming the field of mutation analysis in bacteria. The genetic basis for phenotype alteration can be identified directly by sequencing the entire genome of the mutant and comparing it to the wild-type (WT) genome, thus identifying acquired mutations. A major limitation for this approach is the need for an a-priori sequenced referenc...

Current mediator and wrapper architectures do not have the exibility to scale to multiple wrapped sources, where some sources may be redundant, and some sources may provide incomplete answers to a query. We propose a meta-wrapper component which is capable of handling multiple wrapped sources, in a particular domain, where the multiple sources provide related information. The meta-wrapper makes...

Dynactin is a required cofactor for the minus-end-directed microtubule motor cytoplasmic dynein. Mutations within the highly conserved CAP-Gly domain of dynactin cause neurodegenerative disease. Here, we show that the CAP-Gly domain is necessary to enrich dynactin at the distal end of primary neurons. While the CAP-Gly domain is not required for sustained transport along the axon, we find that ...