onists, azaserine (O-diazoacetyl-L-serine) and DON (6diazo-5-oxo-L-norleucine), in combination with either 6mercaptopurine or 6-thioguanine, synergistically inhibit the growth of a spectrum of transplanted neoplasms of rodents ; these results have been summarized in a recent review (20). In man, although the glutarnine analogs have not proved useful, the purinethiols have achieved some importan...

The effects of 4-aminopyrazolo(3,4-d)pyrimidine (APP), alone or in combination with azaserine, thioguanine, and 6-mercaptopurine, have been tested in Ehrlich ascites carcinoma, Sarcoma 180 ascites, 6C3HED ascites lymphosarcoma, Mecca ascites lymphosarcoma, and TAS ascites carcinoma. The Ehrlich ascites carcinoma is the only tumor of this series which responds to APP. Several combinations of APP...

Polyphenols have been previously shown to sensitize leukemia cell lines to topoisomerase inhibitors. Here, we assess the effects of five polyphenols when used alone and in combination with antimetabolites: methotrexate, 6-mercaptopurine and 5-fluorouracil; in lymphoid and myeloid leukemia cells lines, and non-tumor control cells. The effects of combined treatments were investigated on ATP and g...

Lactate formation from fructose-1,6-diphosphate in homogenates of Sarcoma 180 from mice, given injections intraperitoneally of 6-mercaptopurine, was sharply de creased as compared with that in tumor homogenates of nontreated animals. The activities of aldolase and triosephosphate isomerase, but not of phosphoglyceraldehyde dehydrogenase and phosphoglyceric acid kinase, were significantly lower,...

ase is also described. Radioactivity in plasma, brain, and muscle was determined after administration of 6-mercap topurine, 6-mercaptopurine ribonucleoside, 6-thioguanine, or 6-thioguanine ribonucleoside in mice or rats. At the times chosen for tissue sampling, there were no major differences in brain uptake between the base and ribonu cleoside pairs. Muscle radioactivity ranged from 3 to 7 tim...

Allylsulfides from garlic are chemopreventive agents. Entering cells they are expected to initially interact with glutathione. Accordingly, reaction mechanisms of the product, S-allylthio-glutathione, with model proteins and thiols were analyzed in cell free systems. With glutathionyl, cysteinyl or captopril representing S-allyl aliphatic adducts, the reaction with sulfhydryl groups resulted in...

(CHEST 2002; 122:2238–2241) A 29-year-old man had received a diagnosis of chronic myelogenous leukemia (CML) 6 years before pressentation. He had not undergone bone marrow transplantation because of the lack of an adequate donor. He entered an accelerated phase in 1994 and was treated with oral hydroxyurea and 6-mercaptopurine. The patient developed a dry cough and mild fever in December 2000 a...

Two large studies concluded that AZA started early after diagnosis of Crohn's disease have no late maintenance value. This is contrary to previous studies on 6MP for Crohn's disease and could lead to negating the value of two of the few drugs that have been proven successful. We here outline the many reasons why 6MP remains a valuable drug in the treatment of Crohn's disease.