نتایج جستجو برای: metal binding motif

تعداد نتایج: 633452  

Journal: :The Journal of biological chemistry 2000
U Jakob M Eser J C Bardwell

The chaperone activity of the heat shock protein Hsp33 is regulated by reversible disulfide bond formation. Oxidized Hsp33 is active, and reduced Hsp33 is inactive. We show that zinc binding is essential for the function of this redox switch. Our results reveal that Hps33 contains a new, high affinity (K(a) > 10(17) m(-)(1)), zinc-binding motif in the form Cys-X-Cys-X(27-32)-Cys-X-X-Cys. All fo...

Journal: :Biochemistry 2006
Sabrina Esposito Ilaria Baglivo Gaetano Malgieri Luigi Russo Laura Zaccaro Luca D D'Andrea Marco Mammucari Benedetto Di Blasio Carla Isernia Roberto Fattorusso Paolo V Pedone

Transcriptional factors bearing a Cys(2)His(2) zinc finger were thought to be confined to eukaryotes, but recent studies have suggested their presence also in prokaryotes. In this paper, we report the first complete functional characterization of the DNA binding domain present in the putative Cys(2)His(2) zinc finger-containing prokaryotic transcriptional regulator Ros from Agrobacterium tumefa...

P. atlantica subsp. Kurdica, with the local name of Baneh, is a wild medicinal plant which grows in Kurdistan, Iran.  The identification of resistance gene analogs holds great promise for the development of resistant cultivars. A PCR approach with degenerate primers designed according to conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (R) gene...

Journal: :The Journal of general virology 2001
M A Tortorici P D Ghiringhelli M E Lozano C G Albariño V Romanowski

The arenavirus nucleocapsid protein (N) is a highly basic 63 kDa protein with a dual function during the virus life-cycle. First, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of S RNA, and second it associates with the genomic RNA to form the nucleocapsid. We have expressed the N protein of Junín virus in E. coli and shown ...

Journal: :The Journal of biological chemistry 2007
Mathew Sajish Divya Tiwari Dimple Rananaware Vinay Kumar Nandicoori Balaji Prakash

A major regulatory mechanism evolved by microorganisms to combat stress is the regulation mediated by (p)ppGpp (the stringent response molecule), synthesized and hydrolyzed by Rel proteins. These are divided into bifunctional and monofunctional proteins based on the presence or absence of the hydrolysis activity. Although these proteins require Mg(2+) for (p)ppGpp synthesis, high Mg(2+) was sho...

Journal: :Biochemistry 2000
T C Umland S Q Wei R Craigie D R Davies

Barrier-to-autointegration factor (BAF) is a host cell protein that plays a crucial role in retroviral integration. Preintegration complexes (PICs) stripped of BAF lose their normal integration activity, which can be restored by incubation with purified BAF. BAF bridges double-stranded DNA both intra- and intermolecularly in a non-sequence-specific manner, leading to the formation of a nucleopr...

1998
Weiguo Cao Francis Barany

Metal cofactors (Mg and Mn) modulate both specific DNA binding and strand cleavage in the TaqI endonuclease (Cao, W., Mayer, A. N., and Barany, F. (1995) Biochemistry 34, 2276–2283). This work attempts to establish the structural basis of TaqI-DNA-metal interactions using an affinity cleavage technique. The protein was cleaved by localized hydroxyl radicals generated by oxidizing Fe within the ...

Journal: :Environmental Health Perspectives 2002
Negah Fatemi Bibudhendra Sarkar

Wilson disease is an autosomal recessive disorder of copper metabolism. The Wilson disease protein is a putative copper-transporting P-type ATPase, ATP7B, whose malfunction results in the toxic accumulation of copper in the liver and brain, causing the hepatic and/or neurological symptoms accompanying this disease. The cytosolic N-terminal domain (approximately 70 kDa) of this ATPase comprises ...

Journal: :CHILD`S HEALTH 2022

The scientific review presents the mechanisms of action cytoplasmic miRNAs, namely posttranscriptional silencing: TNRC6-associated mechanism miRNA-mediated mRNA degradation. To write article, information was searched using databases Scopus, Web Science, MedLine, PubMed, Google Scholar, EMBASE, Global Health, Cochrane Library, CyberLeninka. It is known that in cytoplasm cells cases short region,...

Journal: :Journal of virology 1999
M Urabe Y Hasumi A Kume R T Surosky G J Kurtzman K Tobita K Ozawa

The adeno-associated virus (AAV) Rep78 and Rep68 proteins are required for site-specific integration of the AAV genome into the AAVS1 locus (19q13.3-qter) as well as for viral DNA replication. Rep78 and Rep68 bind to the GAGC motif on the inverted terminal repeat (ITR) and cut at the trs (terminal resolution site). A similar reaction is believed to occur in AAVS1 harboring an analogous GAGC mot...

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