The protein product of the CUP1 locus in Cu-resistant Saccharomyces cerevisiae has been purified and characterized. The protein was found to lack the first 8 amino acids predicted by the nucleotide sequence of the gene. The residues removed from the amino-terminal region include 5 hydrophobic residues, two of which are aromatic. The unique amino terminus starting at Gln9 of the putative DNA tra...

PURPOSE To examine in rodent models of retinitis pigmentosa (RP) the expression of the neuroprotectants metallothionein-I and -II and of megalin, an endocytic receptor that mediates their transport into neurons. METHODS Gene and protein expression were analyzed in retinas of rd1 and rds mice and in those of RCS (Royal College of Surgeons) rats of various ages. Glial cell markers (cellular ret...

Neuronal growth-inhibitory factor (GIF), a central-nervous-system-specific metallothionein-like protein, has been isolated by means of an improved isolation procedure from bovine brain. The native protein contains 4-5 Cu+ and 2-2.5 Zn2+, which results in an overall stoichiometry of 6-7 mol metal ions/mol protein. Native Cu, ZN-GIF and the Zn2+ -substituted and Cd2+-substituted metalloforms have...

Evidence suggests a sex difference in intrinsic physiological and diabetic myocardial contractile function related to antioxidant properties of female ovarian hormones. This study was designed to examine the effect of cardiac overexpression of antioxidant metallothionein on intrinsic and diabetic cardiomyocyte function. Weight-matched wild-type (FVB) and metallothionein transgenic mice of both ...

Metallothioneins belong to the group of intracellular, high molecular and cysteine-rich proteins whose content increase with increasing concentration of a heavy metal. Here we applied the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothionein in human blood serum of patient poisoned by lead and/or treated by platinum. The increased...

background and objective: in this study, we explored expression rate, some biomarkers affecting the prognosis of the breast carcinoma, and the relationship between these markers and clinicopathologic features of the disease as well as the relationship between each of these markers through a tissue array technique. materials and methods: this study was an observational and cross-sectional study....

drought stress is one of the serious problems that restricted agronomic plant production worldwide. inmolecular level, the harmful effect of drought stress is mostly caused by producing of large amount ofreactive oxygen species (ros). catalase and metallothionein genes have a crucial role to mope the hydrogenperoxide (h2o2) resulting reducing oxidative damage. in this research the gene expressi...

background: it is proved that testis is sensitive to electromagnetic field (emf) and its damage results in infertility. exposure to emf induces reactive oxygen species production and affects on anti-oxidants defense mechanisms. metallothionein (mt) is a name for a group of low molecular weight (6-7 kda), sulfhydryl rich proteins. expression of mt1 and mt2 genes in testis tissue after emf exposu...

background: zinc (zn) as an important trace element is essential for testicular development and spermatogenesis. molecular mechanism of zn action in the reproductive system may be related to metal binding low-molecular weight proteins, metallothioneins (mt). our objective was to determine the effect of zn on two important isoforms of mt, mt1m and mt1g genes expression on testicular sertoli cell...