Cystinosis is an autosomal recessive disorder characterized by a high intracellular cystine concentration. To establish an in vitro model of this disorder and examine the mechanism of the proximal tubule transport defect seen with elevated intracellular cystine concentrations, rabbit proximal convoluted tubules (PCT) were perfused in vitro. PCTs were loaded with cystine using cystine dimethyl e...

In a screening for antifungal metabolites, two indole compounds of mixed biogenesis, 1H-indole-3-carboxylic acid, 1-(1,1-dimethyl-2-propenyl) methyl ester and 1H-indole-3-carboxylic acid, 1-(2,3-dihydroxy-1,1-dimethylpropyl) methyl ester were isolated from a culture of the basidiomycete Aporpiums caryae. The structural elucidation of these compounds was accomplished by spectroscopic methods.

Poly(ester-acetals) have been prepared from isopropylideneglyceryI azelaaldehydate dimethyl acetal and from methyl azelaaldehydate glycerol acetal. Acid hydrolysis of isopropylideneglyceryl azelaaldehydate led to oligomeric poly (ester-acetals) with six to seven repeating units and carboxylic acid endgroups from which the sodium salt and the methyl ester could be prepared. The polymer sodium sa...

1. The inhibition of pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanylglycine by products and product analogues was studied. 2. The non-competitive nature of the inhibition by the product N-acetyl-l-phenylalanine confirms an ordered release of products, and points to a common mechanism (involving an amino-enzyme) for pepsin-catalysed transpeptidation and hydrolysis reactions...

Four new dicaffeoylquinic acid derivatives and two known 3-caffeoylquinic acid derivatives were isolated from methanol extracts using the aerial parts of Salicornia herbacea. The four new dicaffeoylquinic acid derivatives were established as 3-caffeoyl-5-dihydrocaffeoylquinic acid, 3-caffeoyl-5-dihydrocaffeoylquinic acid methyl ester, 3-caffeoyl-4-dihydrocaffeoylquinic acid methyl ester, and 3,...

The most commonly used method for biodiesel preparation is via transesterification of vegetable oil using alkaline catalysts. Biodiesel yield and oil conversion are affected by operating conditions including the catalyst formulation and concentration. Application of alkaline catalysts can also lead to undesired soap formation. This study evaluated the alkaline catalyst effects on biodiesel yiel...

A direct radioimmunoassay of the methyl ester of urinary and serum 5-hydroxy-3-indole acetic acid is described. The antiserum, raised in a rabbit against a conjugate of bovine serum albumin with 5-hydroxytryptamine hemisuccinamide, contained two antigenic fractions, one binding N-acyl 5-hydroxytryptamine, and the other binding methyl ester of 5-hydroxy-3-indole acetic acid, and N-acyl 5-hydroxy...

General information. All manipulations were performed in a N2 filled drybox or using standard Schlenk techniques. Allyl benzene, methyl vinyl ketone, pchlorostyrene, methyl methacrylate, diethyl diallylmalonate (3), and 1,6-heptadien-4ol (6) were purchased from Aldrich and used without further purification. cis-4Cyclopentene-1,3-diol and (+)-citronellal were purchased from Fluka and used withou...

In this study, the methanolysis process of sunflower oil was investigated to get high methyl esters (biodiesel) content using sodium methoxide. To reach to the best process conditions, central composite design (CCD) through response surface methodology (RSM) was employed. The optimal conditions predicted were the reaction time of 60 min, an excess stoichiometric amount of alcohol to oil ratio o...

Amritotharanam Kashyam, a specific Ayurvedic drug, was the focus of current inquiry to evaluate its efficacy. For liver and digestive-related issues, this medication is suggested. This obtained from standard vendor in Chennai (India), GC-MS analysis carried out according procedure. A few critical biomolecules include benzoic acid, hexadecanoic 6,9-octadecadienoic 9-octadecenoic methyl ester (E)...