An understanding of microbial community structure is an important issue in the field of molecular ecology. The traditional molecular method involves amplification of small subunit ribosomal RNA (SSU rRNA) genes by polymerase chain reaction (PCR). However, PCR-based amplicon approaches are affected by primer bias and chimeras. With the development of high-throughput sequencing technology, unbias...

BACKGROUND Anti-microbial lock solutions (AML), in conjunction with systemic antibiotics, may successfully treat tunnelled haemodialysis catheter-related bloodstream infections (CR-BSI). It is unknown whether AML promote anti-microbial resistance. METHODS This is a retrospective cohort study of all CR-BSI (2003-2006) in our dialysis unit. Controls (n = 265) were treated with systemic vancomyc...

The climate sensitivity of microbe-mediated soil processes such as carbon and nitrogen cycling offers an interesting case for evaluating the corresponding sensitivity of microbial community composition to environmental change. Better understanding of the degree of linkage between functional and compositional stability would contribute to ongoing efforts to build mechanistic models aiming at pre...

We compared three methods used microbial culturing for detection of ureaplasmas in endotracheal aspirate from 500 prematurely born neonates with respiratory disturbances: BioMerieux test, PCR and microbial culturing. Ureaplasmas were detected in respiratory tracts of 79 (16%) newborns. Correlation of the results of culture with those obtained with the BioMerieux kit, culture with PCR and BioMer...

conclusions: even, very low amount of csf-igg with good specificity (90%); could differentiate cases and controls (p = 0.000). the csf-igg test (sensitivity 70%, npv 28.8%) was weak for ruling out the m. pneumonia in cases. positive pcr was rare (2%) in csf of cases and controls but is more reliable for diagnosing the recent m. pneumonia infection. we prefer to assay the csf for both serology a...

background: in this study, the presence of resistance to diclazuril, amprolium+ethopabate and salinomycin, representing some of the commonest anticoccidials in iran’s poultry industry, against three mixed eimeria field isolates were investigated. methods: three eimeria field isolates, collected from typical broiler farms in iran, were propagated once, inoculated to 480 broilers, comprising 30 c...