Microorganism-derived polyene polyketides have been shown to display a variety of biological activities and have attracted great interest due to their structurally intriguing chemical diversity. Two new polyenes were isolated from a culture broth of Streptomyces sp. KMC-002 obtained from a soil sample in an abandoned mine. The structures of these compounds were determined to be α-pyrone-contain...

A new series of novel 3-azabicyclic spiro 1',2',4'-triazolidin-3'-thiones 15-21 catalyzed by potassium superoxide under microwave irradiation have been synthesized and studied for their in vitro microbiological evaluation on clinically isolated bacterial strains namely Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Pseudomonas aeuroginosa and fungal strains namely Aspergillus niger, Ca...

Coaggregating strains of aquatic bacteria were identified by partial 16S rRNA gene sequencing. The coaggregation abilities of four strains of Blastomonas natatoria and one strain of Micrococcus luteus varied with culture age but were always maximum in the stationary phase of growth. Each member of a coaggregating pair carried either a heat- and protease-sensitive protein (lectin) adhesin or a s...

Cell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are different. The release of 7-methylguanine by...

We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA a...

We describe a simple, rapid, sensitive, and highly reproducible assay for lysozyme, with use of concentrated cell suspensions of Micrococcus Iysodeikticus in Tris-buffered glycerol/water (40/60 by vol), pH 7.5. Stored at -20 #{176}C, the cells’ susceptibility to lysozyme remains unaltered over long periods. Almost identical concentration curves were obtained with different aliquots of the same ...

Examination of the effects of prumycin on sensitlve Botrytis cinerea and Sarcina lutea showed that inhibition of protein synthesis in both organisms and reduction in ribonucleic acid synthesis in Botrytis cinerea are among the mechanisms of action. The inhibition of RNA biosynthesis in Botrytis cinerea is probably a secondary effect resulting from the termination of protein synthesis rather tha...

The post-translationally modified peptide antibiotic nisin has been cleaved by a number of proteases and the fragments produced purified, characterised chemically, and assayed for activity in inhibiting the growth of Lactococcus lactis MG1614 and Micrococcus luteus NCDO8166. These results provide information on the importance of different parts of the nisin molecule for its growth-inhibition ac...