Microfluidic systems have significant implications in the field of in vitro cell-based assays and drug screening by virtue of their automation and high content screening capabilities. Here, we present our recent efforts to develop a microfluidic platform for high throughput and high content analysis of cell death event. A group of microfluidic devices for monitoring various cell death parameter...

The precise localization and controlled chemical treatment of structures on a surface are significant challenges for common laboratory technologies. Herein, we introduce a microfluidic-based technology, employing a double-layer microfluidic device, which can trap and localize in situ and ex situ synthesized structures on microfluidic channel surfaces. Crucially, we show how such a device can be...

This review describes the microfluidic techniques developed for the analysis of a single cell. The characteristics of microfluidic (e.g., little sample amount required, high-throughput performance) make this tool suitable to answer and to solve biological questions of interest about a single cell. This review aims to introduce microfluidic related techniques for the isolation, trapping and mani...

The past two decades have seen far-reaching progress in the development of microfluidic systems for use in the chemical and biological sciences. Here we assess the utility of microfluidic reactor technology as a tool in chemical synthesis in both academic research and industrial applications. We highlight the successes and failures of past research in the field and provide a catalogue of chemis...

The development and analysis of the performance of microfluidic components for lab-on-a-chip devices are becoming increasingly important because microfluidic applications are continuing to expand in the fields of biology, nanotechnology, and manufacturing. Therefore, the characterization of fluid behavior at the scales of microand nanometer levels is essential. A variety of microfluidic velocim...

We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of ...

Life scientists are closely working with engineers to solve biological and biomedical problems through the application of engineering tools. For engineers involved in this collaborative work, new knowledge is created in their own disciplines. For example, the science and technology at the interface of biomedical sciences and microfluidics has played a significant role in ushering in recent adva...

Molecular beacons, oligonucleotide probes that fluoresce upon hybridization to a target nucleic acid, can be used in microfluidic devices to detect and quantify nucleic acids in solution as well as inside bacterial cells. Three essential steps towards the development of such devices as integrated microfluidic biosensors using molecular beacons were investigated in the present study. First, expe...

Microfluidics has been the key component for many applications, including biomedical devices, chemical processors, microactuators, and even wearable devices. This technology relies on soft lithography fabrication which requires cleanroom facilities. Although popular, this method is expensive and labor-intensive. Furthermore, current conventional microfluidic chips precludes reconfiguration, mak...

The dawn of the new millennium saw a trend towards the dedicated use of microfluidic devices for process intensification in biotechnology. As the last decade went by, it became evident that this pattern was not a short-lived fad, since the deliverables related to this field of research have been consistently piling-up. The application of process intensification in biotechnology is therefore see...