BACKGROUND Microsatellite analysis of urine sediments has shown promise as a highly sensitive and specific technique for the detection of bladder cancer. However, most studies have been conducted in Western countries with Caucasian subjects. We explored the potential of microsatellite analysis for detecting bladder cancer in Chinese people. METHODS We performed microsatellite analysis of surg...

Due to microsatellite mutations during PCR, stutter patterns may appear in the final PCR product, which hinder us from accurate genotyping microsatellite markers. The existing methods for microsatellite stutter pattern deconvolution required large amount of data. A mathematical model for microsatellite mutations during PCR and an estimation method based on mean field approximation for branching...

We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of polymorp...

Antheraea assama, an economically important and scientifically unexplored Indian wild silkmoth, is unique among saturniid moths. For this species, a total of 87 microsatellite markers was derived from 35 000 expressed sequence tags and a microsatellite-enriched sub-genomic library. Forty individuals collected from Tura and West Garo Hills region of Northeast India were screened for each of thes...

objective(s)marfan syndrome (mfs) is a severe connective tissue disorder withan autosomal dominant inheritance pattern. early diagnosis is critical in mfs. because of the large size of fibrillin-1 gene (fbn1), the uniqueness of mutations, and the absence of genotype-to-phenotype correlations linkage analysis can be very helpful for early diagnosis of mfs. in this study, eight polymorphic marker...

the genetic diversity among 56 almond (prunus dulcis) genotypes was analysed using 35 microsatellite markers and 14 morphological traits. analysis of morphological traits revealed a wide range of variation among the studied genotypes. out of 35 simple sequence repeats (ssrs) markers, 25 were polymorphic, producing 215 alleles that varied from 2 to 16 with an average of 8.76 alleles per locus. r...

iron is one of the most important nutrients in the human diet. according to the high consumption of staple foods such as wheat, the deficiency of iron in these crops would lead to nutritional disorders and related complications. to identify microsatellite markers associated with wheat grain iron content,38iranian prevalent wheat genotypes were assessed using 30 pairs of genomic and est microsat...

bream (abramis brama orientalis) is one of the most commercially valuable fish in the caspian sea. the aim of this study was to compare levels of genetic polymorphism between wild and farmed bream populations using seven microsatellite loci. genetic diversity was investigated by studying samples collected from two regions; chaboksar and the artificial propagation center of guilan province. alle...

this study was carried out to investigate the genetic diversity and population structure of 90 specimens of oxynoemacheilus argyrogramma collected from sepidbarg, gamasiab and ghaleji rivers, in the west of iran. analyses using three microsatellite loci indicated that the average number of alleles in the population was 12, which was well above the reported values for freshwater fishes. the expe...

kutum is considered as one of the anadramous species of the caspian sea. due to continuous population decline of the fish since 1975, iranian fisheries organization started to restock this species. nowadays, there is growing concern over the effects of restocking on natural populations. for this purpose, population structure and genetic variation of this species in the iranian coastline of casp...