Hybrids were produced by crossing female Caspian kutum Rutilus frisii kutum with male grass carp Ctenopharyngodon idella. The genome of eight larvae and parents were studied using microsatellite markers for genetic evaluation and verification. After DNA extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers. Hybridizie...

A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes, while MLP identifies microsatellite instability. Both methods generate unambiguous and highl...

The rational use of valuable fish species from aquaculture is difficult to implement without knowledge the state genetic structure local stocks. Different types DNA markers can be used achieve goals selection and breeding work. a stock rainbow trout (Oncorhynchus mykiss Walbaum, 1792) (Salmoniformes, Salmonidae) farmed in Ukraine was studied using DNA-markers: microsatellite (SSR-markers – simp...

Plant development consists of the initial phase of intensive cell division followed by continuous genome endoreduplication, cell growth, and elongation. The maintenance of genome stability under these conditions is the main task performed by DNA repair and genome surveillance mechanisms. Our previous work showed that the rate of homologous recombination repair in older plants decreases. We hypo...

Starry flounder (Platichthys stellatus) is an important sport and food fish found around the margins of the North Pacific. Aquaculture production of this species in Korea has increased because of its commercial value. Microsatellite DNA markers are a useful DNA-based tool for monitoring the genetic variation of starry flounder populations. In this study, 12 polymorphic microsatellite DNA marker...

Microsatellite markers are used for loss-of-heterozygosity, allelic imbalance and clonality analyses in cancers. Usually, tumor DNA is compared to corresponding normal DNA. However, normal DNA is not always available and can display aberrant allele ratios due to copy number variations in the genome. Moreover, stutter peaks may complicate the analysis. To use microsatellite markers for diagnosis...

AIM Microsatellites are the repeated DNA sequences scattered widely within the genomes and closely linked with many important genes. This study was designed to characterize the changes of microsatellite DNA loss of heterozygosity (LOH) in esophageal carcinogenesis. METHODS Allelic deletions in 32 cases of matched precancerous, cancerous and normal tissues were examined by syringe microdissect...

The purpose of this study was to assess the possibility of prognosis of oral squamous cell carcinoma by microsatellite blood assay. Altered microsatellite DNA in the blood of cancer patients may provide a novel method for tumor detection. DNA from normal and tumor tissues and serum obtained at two time-points (preoperatively and postoperatively) in 20 patients with oral squamous cell carcinoma ...

The main objective of this study was to assess genetic comparison of two migratory forms of Caspian trout Salmo trutta caspius namely fall-run and spring-run. Owing to the lack of information on its genetic differences, 5 microsatellite loci were used for 58 sample analyses. Genomic DNA was extracted from caudal fin using Roche DNA extraction kit and each PCR reaction was performed in a 25 ?l ...

Ulcerative colitis increases colorectal cancer risk by mechanisms that remain incompletely understood. We approached this question determining the genetic and epigenetic profiles of colitis-associated carcinomas (CA-CRC). The findings were compared to Lynch syndrome (LS), a different form predisposition shares importance immunological factors in tumorigenesis. CA-CRCs (n = 27) investigated for ...