The objective of this study was to examine the effect of combining ruminally degradable and undegradable CP sources on ruminal microbial protein synthesis and postruminal N and amino acid (AA) flows in steers. Six steers fitted with ruminal, duodenal, and ileal cannulas were fed diets containing corn silage and high-moisture corn supplemented with urea, soybean meal (SBM), dry corn gluten feed ...

According to the usually accepted concept, the urea removed from the blood by the kidneys is excreted in two forms, chiefly as urea itself, but partly as ammonia. That the ammonia of the urine is formed in the kidneys was demonstrated by Nash and Benedict (1921), in work which has been confirmed by other authors (cf. Peters and Van Slyke, I, p. 373). Evidence from animal experiments in v4vo has...

The objective of this experiment was to determine the capability of ruminant gut tissues to detoxify ammonia-N using short-term incubations of isolated cells in vitro. Ruminal epithelial cells (REC) and duodenal mucosal cells (DMC) were isolated from growing Texel-Polypay ram lambs (n = 4) fed a pelleted forage:concentrate-based diet. Immediately after isolation, primary cells were incubated fo...

1. The rate of transfer of plasma urea-nitrogen to rumen ammonia was measured by infusion of 15NH4Cl and [15N]urea into sheep given brome grass (Bromus inermis) or lucerne (Medicago sativa) pellets. Urea was infused into the rumen or abomasum of two sheep given brome grass in order to increase the concentration of rumen ammonia. 2. From 6.2 to 9.8 g/d of plasma urea-N were transferred to the ru...

The aim of the study was to evaluate effect crude protein (CP) levels in concentrate and Sesbania grandiflora pod meal (SG) supplementation on feed intake, rumen fermentation, methane (CH4) mitigation Thai purebred beef cattle. Four cattle with 100 ± 5.0 kg body weight were used this study. A 2 × factorial experiment a 4 Latin square design conducted, which factor CP 14% 16% dry matter (DM) B s...

milk urea nitrogen (mun) of dairy cows was determined in relation to breed. a total of forty (40) pabna and red chittagong dairy cattle each of twenty (20) were selected to know the milk composition, nutritional status and milk urea nitrogen. live weight of pabna cattle (187±14 kg) was higher than that of red chittagong cattle (174±4 kg). in pabna cattle milk yield (4.94 kg), minerals (0.15%) a...

Lactatingdairy cows (n=177) fed with grass and corn silage ad libitum kept in pasture, were randomly assigned to evaluate how urea nitrogen in plasma and milk can be related to their pregnancy rate. Blood and milk samples were collected on the artificial insemination (AI) day to evaluate plasma urea nitrogen (PUN) and milk urea nitrogen (MUN) as well as progesterone levels, excluding cows with ...

Three ruminally cannulated and multicatheterised lactating dairy cows were used to investigate the effect of different supplement strategies to fresh clover grass on urea and short-chain fatty acid (SCFA) metabolism in a zero-grazing experiment with 24-h blood and ruminal samplings. Fresh clover grass was cut every morning and offered from 0800 to 1500 h. Maize silage was fed at 1530 h. The thr...

the replacement of corn silage with varying levels of ammonia gassed wheat straw on performance, digestibility, fermentation parameters, chewing activities, blood metabolites and profitability of holstein heifers have been evaluated. fifteen holstein heifer with 9-10 months of age were assigned in a completely randomized design with 3 treatment as 1) 36% corn silage, 2) 18% corn silage and 18% ...